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In order to elucidate the effects of dasatinib on the proliferation of lymphocyte subset, dasatinib was added to the culture of peripheral blood mononuclear cells with IL\u20102 (lymphokine\u2010activated killer culture) or a low dose of IL\u20102 with zoledronate (\u03b3\u03b4 T\u2010cell culture). In both culture conditions, NK cells were increased in both percentage and absolute number in the culture with dasatinib compared with control culture without dasatinib. The increase of NK cells was dose dependent of dasatinib in the range of 2\u201325\u2009nM. NK cell cytotoxicity of cultured cells with dasatinib was demonstrated to be superior to control cells without dasatinib in cytotoxicity assay using EGFP\u2010transfected K562 cells as target cells. The present study suggested that lymphocytosis in dasatinib\u2010treated CML patients is at least partly associated with a direct effect of dasatinib to stimulate the proliferation of NK cells. Favourable prognosis in patients with dasatinib\u2010induced lymphocytosis might be associated with the effects of dasatinib to potentiate NK cytotoxicity <jats:italic>in vivo<\/jats:italic>. Copyright \u00a9 2012 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/hon.2034","type":"journal-article","created":{"date-parts":[[2012,10,29]],"date-time":"2012-10-29T17:12:48Z","timestamp":1351530768000},"page":"156-163","source":"Crossref","is-referenced-by-count":20,"title":["Direct effect of dasatinib on proliferation and cytotoxicity of natural killer cells in <i>in vitro<\/i> study"],"prefix":"10.1002","volume":"31","author":[{"given":"Takayoshi","family":"Uchiyama","sequence":"first","affiliation":[{"name":"Laboratory of Hematology and Oncology, Graduate School of Health Sciences Niigata University  Niigata Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoya","family":"Sato","sequence":"additional","affiliation":[{"name":"Laboratory of Hematology and Oncology, Graduate School of Health Sciences Niigata University  Niigata 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