{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T14:17:58Z","timestamp":1781533078305,"version":"3.54.5"},"reference-count":18,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,11,19]],"date-time":"2013-11-19T00:00:00Z","timestamp":1384819200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hematological Oncology"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We compared two strategies for minimal residual disease evaluation of B\u2010cell lymphoproliferative disorders characterized by a variable immunoglobulin heavy chain (<jats:italic>IGH<\/jats:italic>) genes mutation load. Twenty\u2010five samples from chronic lymphocytic leukaemia (<jats:italic>n<\/jats:italic>\u2009=\u200918) or mantle cell lymphoma (<jats:italic>n<\/jats:italic>\u2009=\u20097) patients were analyzed. Based on <jats:italic>IGH<\/jats:italic> variable region genes, 22\/25 samples carried &gt;2% mutations, 20\/25\u2009&gt;\u20095%. In the <jats:italic>IGH<\/jats:italic> joining region genes, 23\/25 samples carried &gt;2% mutations, 18\/25\u2009&gt;\u20095%. Real\u2010time quantitative polymerase chain reaction was performed on IGH genes using two strategies: method A utilizes two patient\u2010specific primers, whereas method B employs one patient\u2010specific and one germline primer, with different positions on the variable, diversity and joining regions. Twenty\u2010three samples (92%) resulted evaluable using method A, only six (24%) by method B. Method B poor performance was specifically evident among mutated <jats:italic>IGH<\/jats:italic> variable\/joining region cases, although no specific mutation load above, which the real\u2010time quantitative polymerase chain reaction failed was found. The molecular strategies for minimal residual disease evaluation should be adapted to the B\u2010cell receptor features of the disease investigated. Copyright \u00a9 2013 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/hon.2095","type":"journal-article","created":{"date-parts":[[2013,11,20]],"date-time":"2013-11-20T02:48:41Z","timestamp":1384915721000},"page":"133-138","source":"Crossref","is-referenced-by-count":11,"title":["Comparison of two real\u2010time quantitative polymerase chain reaction strategies for minimal residual disease evaluation in lymphoproliferative disorders: correlation between immunoglobulin gene mutation load and real\u2010time quantitative polymerase chain reaction performance"],"prefix":"10.1002","volume":"32","author":[{"given":"Irene","family":"Della Starza","sequence":"first","affiliation":[{"name":"Department of Cellular Biotechnologies and Hematology \u201cSapienza\u201d University  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marzia","family":"Cavalli","sequence":"additional","affiliation":[{"name":"Department of Cellular Biotechnologies and Hematology \u201cSapienza\u201d University  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilaria","family":"Del Giudice","sequence":"additional","affiliation":[{"name":"Department of Cellular Biotechnologies and Hematology \u201cSapienza\u201d University  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Barbero","sequence":"additional","affiliation":[{"name":"Department of Molecular Biotechnologies and Health Sciences University of Turin  Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Mantoan","sequence":"additional","affiliation":[{"name":"Department of Molecular Biotechnologies and Health Sciences University of Turin  Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa","family":"Genuardi","sequence":"additional","affiliation":[{"name":"Department of Molecular Biotechnologies and Health Sciences University of Turin  Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Urbano","sequence":"additional","affiliation":[{"name":"Department of Molecular Biotechnologies and Health Sciences University of Turin  Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Mannu","sequence":"additional","affiliation":[{"name":"Department of Experimental, Diagnostic and Specialty Medicine University of Bologna  Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Gazzola","sequence":"additional","affiliation":[{"name":"Department of Experimental, Diagnostic and Specialty Medicine University of Bologna  Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Ciabatti","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine, UO Hematology University of Pisa  Pisa 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