{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T19:57:54Z","timestamp":1792094274932,"version":"4.4.0"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,10,5]],"date-time":"2016-10-05T00:00:00Z","timestamp":1475625600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Nature Sciences Foundation of China","doi-asserted-by":"crossref","award":["81470359"],"award-info":[{"award-number":["81470359"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hematological Oncology"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The loss of PRDM1 expression is common in extranodal NK\/T\u2010cell lymphoma, nasal type (EN\u2010NK\/T\u2010NT), but the role of promoter methylation in silencing PRDM1 expression remains unclear. Hence, we performed pyrosequencing analysis to evaluate the promoter methylation of PRDM1 gene in vivo and in vitro, to analyze the association between methylation and its expression, and to assess cellular effects of PRDM1 reexpression. The promoter hypermethylation of PRDM1 gene was detected in 11 of 25 EN\u2010NK\/T\u2010NT cases (44.0%) and NK92 and NKL cells. The promoter hypermethylation of PRDM1 was significantly correlated with PRDM1 expression in vivo and in vitro, predominantly contributing to the loss of PRDM1 expression compared with genetic deletion and aberrant expression of miR\u2010223 in EN\u2010NK\/T\u2010NT. PRDM1 expression was significantly restored by demethylation treatment, which induced cell proliferation suppression, cell cycle arrest, and apoptosis increase. We also found that PRDM1 reexpression could downregulate the expression of Ets\u20101, T\u2010bet, granzyme B, and c\u2010<jats:italic>myc<\/jats:italic>. Our findings demonstrated that the promoter hypermethylation of PRDM1 harbored a predominant role in the downregulation of PRDM1 expression, significantly affecting the biological behavior of tumor cells in EN\u2010NK\/T\u2010NT.<\/jats:p>","DOI":"10.1002\/hon.2362","type":"journal-article","created":{"date-parts":[[2016,10,12]],"date-time":"2016-10-12T17:13:39Z","timestamp":1476292419000},"page":"645-654","source":"Crossref","is-referenced-by-count":28,"title":["Hypermethylation of PRDM1\/Blimp\u20101 promoter in extranodal NK\/T\u2010cell lymphoma, nasal type: an evidence of predominant role in its downregulation"],"prefix":"10.1002","volume":"35","author":[{"given":"Zhang","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Pathology Peking University First Hospital  Beijing 100034 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Pathology Peking University 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