{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T19:21:25Z","timestamp":1784056885848,"version":"3.55.0"},"reference-count":19,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,9,15]],"date-time":"2019-09-15T00:00:00Z","timestamp":1568505600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003787","name":"Natural Science Foundation of Hebei Province","doi-asserted-by":"publisher","award":["H2019B72003"],"award-info":[{"award-number":["H2019B72003"]}],"id":[{"id":"10.13039\/501100003787","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Hematological Oncology"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Ninety percent of relapse\/refractory B\u2010cell acute lymphatic leukemia (R\/R B\u2010ALL) patients can achieve complete remission (CR) after CD19\u2010targeting chimeric antigen receptor T (CAR\u2010T) cell therapy. However, around 50% of them relapse in 1 year. Persistent CAR\u2010T cell engraftment is considered as the key to remain durable remission. Here, we initiated a phase I study to treat 10 pediatric B\u2010ALL patients using a CD19\u2010targeted second generation CAR with a 4\u20101BB intracellular costimulatory domain. All patients received a standard fludarabine and cyclophosphamide (FC) preconditioning regiment, followed by a CAR\u2010T infusion with a median number of 0.5 (0.3\u20101.58) \u00d7 10\n                    <jats:sup>6<\/jats:sup>\n                    CAR+ T cells\/kg. The pretreatment tumor burdens were high with a median bone marrow (BM) blasts percentage of 59.2% (7.31%\u201086.2%), excluding one patient only with brain infiltration of leukemia cells (0% BM blasts). The initial CR rate was 80% (n = 8\/10). Four patients (40%) experienced serious (grade &gt; 2) cytokine release syndrome (CRS) and three patients (30%) with obvious neurotoxicity. Monthly assessments of CD19+ minimal residual disease (MRD) and CAR\u2010T engraftment demonstrated the anti\u2010CD19 activity of long\u2010term engrafted CAR\u2010T cell clones in one patient for more than 2 years.\n                  <\/jats:p>","DOI":"10.1002\/hon.2672","type":"journal-article","created":{"date-parts":[[2019,8,29]],"date-time":"2019-08-29T13:58:11Z","timestamp":1567087091000},"page":"601-608","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["Evidence of long\u2010lasting anti\u2010CD19 activity of engrafted CD19 chimeric antigen receptor\u2013modified T cells in a phase I study targeting pediatrics with acute lymphoblastic leukemia"],"prefix":"10.1002","volume":"37","author":[{"given":"Futian","family":"Ma","sequence":"first","affiliation":[{"name":"Department of Pediatrics Hematology\u2010Oncology The Second Hospital of Hebei Medical University  Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1094-2248","authenticated-orcid":false,"given":"Jin\u2010Yuan","family":"Ho","sequence":"additional","affiliation":[{"name":"CAR\u2010T Research Center Hebei Senlang Biotechnology Co., Ltd.  Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Hematology\u2010Oncology The Second Hospital of Hebei Medical University  Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Xuan","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Hematology\u2010Oncology The Second Hospital of Hebei Medical University  Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoli","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Hematology\u2010Oncology The Second Hospital of Hebei Medical University  Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinglong","family":"Wang","sequence":"additional","affiliation":[{"name":"CAR\u2010T Research Center Hebei Senlang Biotechnology Co., Ltd.  Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Wang","sequence":"additional","affiliation":[{"name":"CAR\u2010T Research Center Hebei Senlang Biotechnology Co., Ltd.  Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Liu","sequence":"additional","affiliation":[{"name":"CAR\u2010T Research Center Hebei Senlang Biotechnology Co., Ltd.  Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Ba","sequence":"additional","affiliation":[{"name":"CAR\u2010T Research Center Hebei Senlang Biotechnology Co., Ltd.  Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yizhuo","family":"Wang","sequence":"additional","affiliation":[{"name":"CAR\u2010T Research Center Hebei Senlang Biotechnology Co., Ltd.  Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianmin","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Hematology The Second Hospital of Hebei Medical University  Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianqiang","family":"Li","sequence":"additional","affiliation":[{"name":"CAR\u2010T Research Center Hebei Senlang Biotechnology Co., Ltd.  Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,9,15]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1407222"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3002842"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1215134"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3005930"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12185-013-1479-5"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1709919"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI85309"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4441"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(14)61403-3"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrclinonc.2015.38"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI87366"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-08-519413"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-018-0571-y"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0178-z"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI125423"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/CJI.0b013e318270dec7"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2015.247"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-018-0010-1"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.99048"}],"container-title":["Hematological Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/hon.2672","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/hon.2672","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/hon.2672","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T23:17:32Z","timestamp":1694128652000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/hon.2672"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,15]]},"references-count":19,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2019,12]]}},"alternative-id":["10.1002\/hon.2672"],"URL":"https:\/\/doi.org\/10.1002\/hon.2672","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/HON.2672\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/HON.2672\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/HON.2672\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/HON.2672\/v2\/response1","asserted-by":"object"}]},"ISSN":["0278-0232","1099-1069"],"issn-type":[{"value":"0278-0232","type":"print"},{"value":"1099-1069","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9,15]]},"assertion":[{"value":"2019-01-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-09-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}