{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:10:18Z","timestamp":1772118618015,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,11,11]],"date-time":"2019-11-11T00:00:00Z","timestamp":1573430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7143183"],"award-info":[{"award-number":["7143183"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Hematological Oncology"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Immortalized cell lines are useful for deciphering the pathogenesis of acute leukemia and developing novel therapeutic agents against this malignancy. In this study, a new human myeloid leukemia cell line YBT\u20105 was established. After more than 1\u2010year cultivation from the bone marrow of a patient with acute monocytic leukemia, YBT cell line was established. Then a subclone, YBT\u20105, was isolated from YBT using single cell sorting. Morphological and cytogenetical characterizations of the YBT\u20105 cell line were determined by cytochemical staining, flow cytometry analysis, and karyotype analysis. Molecular features were identified by transcriptomic analysis and reverse transcription\u2013polymerase chain reaction. To establish a tumor model, 5 \u00d7 10\n                    <jats:sup>6<\/jats:sup>\n                    YBT\u20105 cells were injected subcutaneously in nonobese diabetic\/severe combined immune\u2010deficiency (NOD\/SCID) mice. DOT1L has been proposed as a potential therapeutic target for KMT2A\u2010related leukemia; therefore, to explore the potential application of this new cell line, its sensitivity to a specific DOT1L inhibitor, EPZ004777 was measured ex vivo. The growth of YBT\u20105 does not depend on granulocyte\u2010macrophage colony\u2010stimulating factor. Cytochemical staining showed that \u03b1\u2010naphthyl acetate esterase staining was positive and partially inhibited by sodium fluoride, while peroxidase staining was negative. Flow cytometry analysis of YBT\u20105 cells showed positive myeloid and monocytic markers.\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/dict.cn\/karyotype\">Karyotype analysis of YBT\u20105 showed<\/jats:ext-link>\n                    48,XY,+8,+8. The breakpoints between KMT2A exon 10 and exon 11 (KMT2A exon 10\/11) and MLLT3 exon 5 and exon 6 (MLLT3 exon 5\/6) were identified, which was different from all known breakpoint locations, and a novel fusion transcript KMT2A exon 10\/MLLT3 exon 6 was formed. A tumor model was established successfully in NOD\/SCID mice. EPZ004777 could inhibit the proliferation and induce the differentiation of YBT\u20105 cells. Therefore, a new acute monocytic leukemia cell line with clear biological and molecular features was established and may be used in the research and development of new agents targeting KMT2A\u2010associated leukemia.\n                  <\/jats:p>","DOI":"10.1002\/hon.2686","type":"journal-article","created":{"date-parts":[[2019,11,8]],"date-time":"2019-11-08T03:15:10Z","timestamp":1573182910000},"page":"617-625","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Establishment and characterization of a DOT1L inhibitor\u2010sensitive human acute monocytic leukemia cell line YBT\u20105 with a novel KMT2A\u2010MLLT3 fusion"],"prefix":"10.1002","volume":"37","author":[{"given":"Zhenhua","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Hematology Peking University First Hospital  Beijing 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