{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T05:53:13Z","timestamp":1781502793019,"version":"3.54.1"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,8,28]],"date-time":"2020-08-28T00:00:00Z","timestamp":1598572800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2020,8,28]],"date-time":"2020-08-28T00:00:00Z","timestamp":1598572800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Hematological Oncology"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Minimal residual disease (MRD) assessment is of high clinical relevance in patients with mantle cell lymphoma (MCL). In mature B\u2010cell malignancies, the presence of somatic hypermutations (SHM) in Variable\u2010Diversity\u2010Joining Heavy chain (VDJH) rearrangements leads to frequent mismatches between primers, probes, and the target, thus impairing tumor cells quantification. Alternative targets, such as immunoglobulin kappa\u2010deleting\u2010element (\n                    <jats:italic>IGK\u2010Kde<\/jats:italic>\n                    ) rearrangements, might be suitable for MRD detection. We aimed at evaluating the applicability of\n                    <jats:italic>IGK\u2010Kde<\/jats:italic>\n                    rearrangements for MRD quantification in MCL patients by real\u2010time quantitative polymerase chain reaction (RQ\u2010PCR)\/digital\u2010droplet\u2010PCR (ddPCR).\n                    <jats:italic>IGK<\/jats:italic>\n                    screening was performed on bone marrow samples from two cohorts: the first from Turin (22 patients enrolled in the FIL\u2010MCL0208 trial, NCT02354313) and the second from Rome (15 patients).\n                    <jats:italic>IGK\u2010Kde<\/jats:italic>\n                    rearrangements were found in 76% (28\/37) of cases, representing the sole molecular marker in 73% (8\/11) of\n                    <jats:italic>IGH\u2010BCL1\/IGH<\/jats:italic>\n                    negative cases. MRD RQ\u2010PCR monitoring was possible in 57% (16\/28) of cases, showing a 100% concordance with the conventional targets. However, the frequent background amplification affected the sensitivity of the assay, that was lower in MCL compared to acute lymphoblastic leukemia and in line with multiple myeloma published results. ddPCR had a good concordance with RQ\u2010PCR and it might help to identify false positive\/negative results. From a clinical perspective, we suggest that\n                    <jats:italic>IGK\u2010Kde<\/jats:italic>\n                    can be a candidate target for MRD monitoring and deserves a validation of its predictive value in prospective MCL series.\n                  <\/jats:p>","DOI":"10.1002\/hon.2792","type":"journal-article","created":{"date-parts":[[2020,8,20]],"date-time":"2020-08-20T11:46:05Z","timestamp":1597923965000},"page":"698-704","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Immunoglobulin kappa deleting element rearrangements are candidate targets for minimal residual disease evaluation in mantle cell lymphoma"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7909-8642","authenticated-orcid":false,"given":"Irene","family":"Della Starza","sequence":"first","affiliation":[{"name":"Hematology Department of Translational and Precision Medicine \u201cSapienza\u201d University of Roma  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia Anna","family":"De Novi","sequence":"additional","affiliation":[{"name":"Hematology Department of Translational and Precision Medicine \u201cSapienza\u201d University of Roma  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marzia","family":"Cavalli","sequence":"additional","affiliation":[{"name":"Hematology Department of Translational and Precision Medicine \u201cSapienza\u201d University of Roma  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noemi","family":"Novelli","sequence":"additional","affiliation":[{"name":"Hematology Department of Translational and Precision Medicine \u201cSapienza\u201d University of Roma  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Soscia","sequence":"additional","affiliation":[{"name":"Hematology Department of Translational and Precision Medicine \u201cSapienza\u201d University of Roma  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa","family":"Genuardi","sequence":"additional","affiliation":[{"name":"Division of Hematology Department of Molecular Biotechnologies and Health Sciences University of Torino  Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Mantoan","sequence":"additional","affiliation":[{"name":"Division of Hematology Department of Molecular Biotechnologies and Health Sciences University of Torino  Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Drandi","sequence":"additional","affiliation":[{"name":"Division of Hematology Department of Molecular Biotechnologies and Health Sciences University of Torino  Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Ferrante","sequence":"additional","affiliation":[{"name":"Division of Hematology Department of Molecular Biotechnologies and Health Sciences University of Torino  Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luigia","family":"Monitillo","sequence":"additional","affiliation":[{"name":"Division of Hematology Department of Molecular Biotechnologies and Health Sciences University of Torino  Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Barbero","sequence":"additional","affiliation":[{"name":"Division of Hematology Department of Molecular Biotechnologies and Health Sciences University of Torino  Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Ciabatti","sequence":"additional","affiliation":[{"name":"Division of Hematology Department of Oncology Santa Chiara Hospital  Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanna","family":"Grassi","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnologies University of Siena  Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riccardo","family":"Bomben","sequence":"additional","affiliation":[{"name":"Clinical and Experimental Onco\u2010Haematology Unit Centro di Riferimento Oncologico, I.R.C.C.S.  Aviano Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimo","family":"Degan","sequence":"additional","affiliation":[{"name":"Clinical and Experimental Onco\u2010Haematology Unit Centro di Riferimento Oncologico, I.R.C.C.S.  Aviano Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valter","family":"Gattei","sequence":"additional","affiliation":[{"name":"Clinical and Experimental Onco\u2010Haematology Unit Centro di Riferimento Oncologico, I.R.C.C.S.  Aviano Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Galimberti","sequence":"additional","affiliation":[{"name":"Division of Hematology Department of Oncology Santa Chiara Hospital  Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice","family":"Di Rocco","sequence":"additional","affiliation":[{"name":"Hematology Department of Translational and Precision Medicine \u201cSapienza\u201d University of Roma  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maurizio","family":"Martelli","sequence":"additional","affiliation":[{"name":"Hematology Department of Translational and Precision Medicine \u201cSapienza\u201d University of Roma  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Cortelazzo","sequence":"additional","affiliation":[{"name":"Hematology, Medical Oncology and Hematology Division \u201cIstituto Clinico Humanitas Gavazzeni\u201d  Bergamo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Guarini","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine \u201cSapienza\u201d University of Roma  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin","family":"Fo\u00e0","sequence":"additional","affiliation":[{"name":"Hematology Department of Translational and Precision Medicine \u201cSapienza\u201d University of Roma  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Ladetto","sequence":"additional","affiliation":[{"name":"Division of Hematology Azienda Ospedaliera SS Antonio e Biagio e Cesare Arrigo  Alessandria Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"Ferrero","sequence":"additional","affiliation":[{"name":"Division of Hematology Department of Molecular Biotechnologies and Health Sciences University of Torino  Torino Italy"},{"name":"Division of Hematology AOU \u201cCitt\u00e0 della Salute e della Scienza di Torino\u201d  Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilaria","family":"Del Giudice","sequence":"additional","affiliation":[{"name":"Hematology Department of Translational and Precision Medicine \u201cSapienza\u201d University of Roma  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Fondazione Italiana Linfomi (FIL) MRD 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