{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:10:42Z","timestamp":1772118642021,"version":"3.50.1"},"reference-count":50,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,12,23]],"date-time":"2020-12-23T00:00:00Z","timestamp":1608681600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2019M661305"],"award-info":[{"award-number":["2019M661305"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Hematological Oncology"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    T\u2010cell acute lymphoblastic leukemia (T\u2010ALL) is an aggressive malignancy. Understanding of the molecular pathogenesis may lead to novel therapeutic targets. Rapamycin, the mammalian target of rapamycin (mTOR) inhibitor, showed inhibitory effects on T\u2010ALL cells. In this study, we showed that rapamycin significantly reduced\n                    <jats:italic>MYCN<\/jats:italic>\n                    mRNA and protein in a concentration\u2010dependent manner in T\u2010ALL cells. Selective knockdown of\n                    <jats:italic>MYCN<\/jats:italic>\n                    by small interfering RNA had similar effects to rapamycin to inhibit T\u2010ALL proliferation and colony formation and to induce G1\u2010phase cell\u2010cycle arrest and apoptosis. The inhibitory effects of rapamycin and\n                    <jats:italic>MYCN<\/jats:italic>\n                    depletion were also found in a Molt\u20104 xenograft model. Rapamycin and\n                    <jats:italic>MYCN<\/jats:italic>\n                    inhibition suppressed both Wnt\/\u03b2\u2010catenin and mTOR signaling pathways. The results suggest the effects of rapamycin on adult T\u2010ALL is likely mediated by downregulation of\n                    <jats:italic>MYCN<\/jats:italic>\n                    . The findings suggest\n                    <jats:italic>MYCN<\/jats:italic>\n                    a potential target for the treatment of adult T\u2010ALL. Additionally, dual targeting of mTOR and Wnt\/\u03b2\u2010catenin pathways may represent a novel strategy in the treatment of adult T\u2010ALL.\n                  <\/jats:p>","DOI":"10.1002\/hon.2831","type":"journal-article","created":{"date-parts":[[2020,12,10]],"date-time":"2020-12-10T18:03:26Z","timestamp":1607623406000},"page":"222-230","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Growth inhibition and suppression of the mTOR and Wnt\/\u03b2\u2010catenin pathways in T\u2010acute lymphoblastic leukemia by rapamycin and\n                    <i>MYCN<\/i>\n                    depletion"],"prefix":"10.1002","volume":"39","author":[{"given":"Desheng","family":"Kong","sequence":"first","affiliation":[{"name":"Department of Hematology The Fourth Affiliated Hospital Harbin Medical University  Harbin 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