{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:57:18Z","timestamp":1761058638831},"reference-count":23,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2007,3,6]],"date-time":"2007-03-06T00:00:00Z","timestamp":1173139200000},"content-version":"vor","delay-in-days":6030,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hematological Oncology"],"published-print":{"date-parts":[[1990,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A panel of 10 monoclonal antibodies reactive with formalin\u2010resistant epitopes was applied to characterize the giant cells of the Reed\u2010Sternberg, Hodgkin, and lacunar cell types in diagnostic biopsies from the German Hodgkin Trial. The 94 tissue samples examined had been sent to the Reference Center by 44 different laboratories which made the initial diagnoses. A board of four referees re\u2010classified the primary diagnoses established by the 44 different histopathologists, providing subtyping of Nodular Sclerosis according to Bennett <jats:italic>et al.<\/jats:italic> (1985, 1989). Only cases with unequivocal agreement among the referees and which satisfied standards of fixation and embedding were included. Giant cells stained positively with: Ber\u2010H2 in 92 per cent (81\/88), LN\u20102 in 86.4 per cent (76\/88), and DAKO\u2010M1 in 72.2 per cent (64\/88) of cases in which the lymphocyte predominance type was not included. Positive staining was quite rare with the other seven monoclonal antibodies. No significant difference in reactivity was revealed between giant cells of Reed\u2010Sternberg, lacunar or Hodgkin cell types. Approximately one third of the cases were negative for at least one of the markers, Ber\u2010H2, LN\u20102 or DAKO\u2010M1. The lymphocytic and histiocytic cells in lymphocyte predominance Hodgkin's disease displayed a distinctive staining pattern with positivity for B\u2010cell markers, whereas DAKO\u2010M1 and Ber\u2010H2 were predominantly negative.<\/jats:p><jats:p>Finally, we found that randomly collected blocks of primary biopsies permit reliable immunostaining by this panel of monoclonal antibodies, since our results agree with results from the literature obtained from biopsies processed according to more uniform protocols for fixation and embedding.<\/jats:p>","DOI":"10.1002\/hon.2900080502","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T19:05:23Z","timestamp":1171911923000},"page":"241-250","source":"Crossref","is-referenced-by-count":8,"title":["Characterization of giant cells in Hodgkin's lymphomas by immunohistochemistry applied to randomly collected diagnostic biopsies from the german Hodgkin trial"],"prefix":"10.1002","volume":"8","author":[{"given":"M.","family":"Werner","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Georgii","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Bernhards","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"H\u00fcbner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. W.","family":"Schwarze","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Fischer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,3,6]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-198804000-00002"},{"key":"e_1_2_1_3_1","first-page":"15","volume-title":"The Cytobiology of Leukaemias and Lymphomas","author":"Bennett M. H.","year":"1985"},{"key":"e_1_2_1_4_1","volume-title":"Progress in Surgical Pathology","author":"Bennett M. H.","year":"1989"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-198801000-00002"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1985.200"},{"key":"e_1_2_1_7_1","first-page":"508","article-title":"An evaluation of the utility of antigranulocyte and anti\u2010leukocyte monoclonal antibodies in the diagnosis of Hodgkin's disease","volume":"123","author":"Dorfman R. F.","year":"1986","journal-title":"Am. J. Pathol."},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.4049\/jimmunol.133.2.1028","article-title":"Two new monoclonal antibodies (LN\u20101, LN\u20102) reactive in B5 fixed, paraffin embedded tissues with follicular center and mantle zone human B\u2010lymphocytes and derived tumors","volume":"133","author":"Epstein A. L.","year":"1984","journal-title":"J. Immunol."},{"key":"e_1_2_1_9_1","unstructured":"Georgii A. Fischer R. H\u00fcbner K. Schwarze E. W. Bernhards J. (1990).Histopathologische Klassifikation von Hodgkin\u2010Lymphomen. Bericht der Referenz\u2010Pathologie der Deutschen Hodgkin\u2010Studie.Pathologe in press."},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.1988.tb02021.x"},{"key":"e_1_2_1_11_1","first-page":"1311","article-title":"Report of the Nomenclature Committee","volume":"26","author":"Lukes R. J.","year":"1966","journal-title":"Cancer Res."},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00431390"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.39.4.399"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.40.12.1405"},{"key":"e_1_2_1_15_1","first-page":"244","article-title":"Leu\u2010M1\u2014a marker for Reed\u2010Sternberg cells in Hodgkin's disease","volume":"119","author":"Pinkus G. S.","year":"1985","journal-title":"Am. J. Pathol."},{"key":"e_1_2_1_16_1","first-page":"1","article-title":"Hodgkin's disease, lymphocyte predominant type, nodular\u2014a distinct entity? Unique staining profile for L & H variants of Reed\u2010Sternberg cells to leucocyte common antigen, granulocyte specific antigen and B cell specific antigen","volume":"118","author":"Pinkus G. S.","year":"1985","journal-title":"Am. J. Pathol."},{"key":"e_1_2_1_17_1","first-page":"418","article-title":"Monoclonal antibodies (MT1, MT2, MB1, MB2, MB3) reactive with leukocyte subsets in paraffin\u2010embedded tissue sections","volume":"127","author":"Poppema S.","year":"1987","journal-title":"Am. J. Pathol."},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19890515)63:10<2030::AID-CNCR2820631027>3.0.CO;2-P"},{"key":"e_1_2_1_19_1","doi-asserted-by":"crossref","first-page":"1678","DOI":"10.1182\/blood.V74.5.1678.1678","article-title":"Ber\u2010H2: A new high avidity monoclonal antibody to a formol resistant epitope of the Ki\u20101 (CD 30) antigen. Immunohistochemical and immunobiochemical characterization","volume":"74","author":"Schwarting R.","year":"1989","journal-title":"Blood"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19860601)57:11<2135::AID-CNCR2820571109>3.0.CO;2-N"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910290310"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910300411"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/86.3.292"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198311243092102"}],"container-title":["Hematological Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fhon.2900080502","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/hon.2900080502","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T02:05:58Z","timestamp":1698026758000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/hon.2900080502"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,9]]},"references-count":23,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1990,9]]}},"alternative-id":["10.1002\/hon.2900080502"],"URL":"https:\/\/doi.org\/10.1002\/hon.2900080502","archive":["Portico"],"relation":{},"ISSN":["0278-0232","1099-1069"],"issn-type":[{"value":"0278-0232","type":"print"},{"value":"1099-1069","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,9]]}}}