{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T06:20:35Z","timestamp":1788848435206,"version":"build-2803163510"},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,4,22]],"date-time":"2022-04-22T00:00:00Z","timestamp":1650585600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Hematological Oncology"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Epstein\u2010Barr virus (EBV), characterized as an omnipresent virus, has been found able to infect NK cells and leads to NK\u2010cell type EBV\u2010positive lymphoproliferative diseases (EBV\u2010NK\u2010LPDs). We retrospective analyzed 202 EBV\u2010NK\u2010LPDs (including 64 CAEBV\u2010NK, 27 aggressive natural killer\u2010cell leukemia (ANKL), and 111 extranodal NK\/T\u2010cell lymphoma (ENKTL)) patients' relationships between EBV DNA copies laboratory test results and clinical features. In CAEBV\u2010NK cohort, EBV DNA loads in either plasma or PBMCs had significant differences between the active state and the inactive state. Receiver operating characteristic curves were used to measure the diagnosis accuracy of EBV DNA copies. After comparing the area under the curve, EBV DNA loads in plasma had significantly higher accuracy in distinguishing disease activation than in PBMCs. Therefore, we propose redefining CAEBV\u2010NK diagnosis criteria as increased EBV DNA copies in plasma (over 7.1\u00a0\u00d7\u00a010\n                    <jats:sup>2<\/jats:sup>\n                    copies\/ml) instead of in peripheral blood. In ANKL and ENKTL cohorts, patients who received effective therapy had significantly lower EBV DNA copies in plasma &amp; PBMCs than in those with ineffective therapy. The significant and consistent decline indicated EBV DNA loads in plasma being a more sensitive biomarker in monitoring EBV\u2010NK\u2010LPDs therapy responses. Hemophagocytic lymphohistiocytosis (HLH) can occur secondary to EBV\u2010NK\u2010LPDs, mostly associated with a poor prognosis, so we try to estimate the combination of HLH by monitoring EBV DNA copies. When comparing the Receiver operating characteristic curves of EBV DNA copies, EBV DNA loads in plasma had higher diagnosis accuracy. When the copies level over 4.16\u00a0\u00d7\u00a010\n                    <jats:sup>3<\/jats:sup>\n                    copies\/ml, it might indicate combining with HLH.\n                  <\/jats:p>","DOI":"10.1002\/hon.2998","type":"journal-article","created":{"date-parts":[[2022,4,11]],"date-time":"2022-04-11T18:50:16Z","timestamp":1649703016000},"page":"381-389","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["The superiority of Epstein\u2010Barr virus DNA in plasma over in peripheral blood mononuclear cells for monitoring EBV\u2010positive NK\u2010cell lymphoproliferative diseases"],"prefix":"10.1002","volume":"40","author":[{"given":"Miao","family":"Zheng","sequence":"first","affiliation":[{"name":"Department of Hematology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8276-7330","authenticated-orcid":false,"given":"Yuhan","family":"Bao","sequence":"additional","affiliation":[{"name":"Department of Hematology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiachen","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Hematology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaxian","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Hematology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan 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University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianfeng","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Hematology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,4,22]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-032414-112326"},{"key":"e_1_2_11_3_1","first-page":"2655","volume-title":"Fields Virology","author":"Rickinson AB","year":"2007"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdp064"},{"key":"e_1_2_11_5_1","volume-title":"WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues","author":"Swerdlow 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