{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T22:06:52Z","timestamp":1767046012107,"version":"3.40.5"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,9,30]],"date-time":"2022-09-30T00:00:00Z","timestamp":1664496000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005010","name":"Associazione Italiana per la Ricerca sul Cancro","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005010","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Hematological Oncology"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Chronic lymphocytic leukemia (CLL), the most common leukemia in the western countries, is characterized by immunosuppression due to disease itself and cytotoxic treatments. Since the beginning of COVID\u201019 pandemic, patients with CLL appear to be a vulnerable population. In addition, phase III mRNA vaccine trials did not provide information about the efficacy in immunocomprised population. In CLL, the antibody\u2010mediated response to SARS\u2010CoV\u20102 vaccine is impaired. The goal of this study was to evaluate the effects of SARS\u2010CoV\u20102 vaccination on humoral immune response and on cellular immunity in CLL patients. Humoral immune response to BNT162b2 messenger RNA COVID\u201019 vaccine was evaluated in 44 CLL patients comprising 20 treatment\u2010na\u00efve, 14 under treatment with ibrutinib and 10 in follow\u2010up after completion of therapy. A positive serological response to SARS\u2010CoV\u20102 vaccination with IgG titers higher than 13\u00a0UA\/ml was detected in 54.6% of CLL patients with a higher response in patients who obtained remission after treatment. Reduced antibody response was detected in patients under ibrutinib treatment. T\u2010cell response to overlapping pool of peptides representing the spike region was assessed in paired CLL samples collected before and after 1\u00a0month from the second dose of COVID\u201019 vaccine in treatment\u2010na\u00efve and ibrutinib\u2010treated CLL patients using cytokine secretion assay. Both CD3+ CD4+ and CD3+ CD8+ T cells are able to mount a cellular response to spike peptides with secretion of IFN\u03b3 and TNF\u03b1 before and after vaccination in both treatment na\u00efve and ibrutinib\u2010treated patients and this cellular immune response is independent by COVID\u201019 vaccination. Collectively, T cell response to spike peptides appeared more blunted in CLL patients under treatment with ibrutinib compared to untreated ones. Our study supports the need for optimization of vaccination strategy to achieve an adequate immune response keeping strict preventive measures by CLL patients against COVID\u201019.<\/jats:p>","DOI":"10.1002\/hon.3077","type":"journal-article","created":{"date-parts":[[2022,9,26]],"date-time":"2022-09-26T02:19:56Z","timestamp":1664158796000},"page":"120-127","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Effects of the BTN162b2 mRNA COVID\u201019 vaccine in humoral and cellular immunity in patients with chronic lymphocytic leukemia"],"prefix":"10.1002","volume":"41","author":[{"given":"Stefania","family":"Fiorcari","sequence":"first","affiliation":[{"name":"Department of Medical and Surgical Sciences Section of Hematology University of Modena and Reggio Emilia  Modena Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudio Giacinto","family":"Atene","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences Section of Hematology University of Modena and Reggio Emilia  Modena Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3518-2006","authenticated-orcid":false,"given":"Rossana","family":"Maffei","sequence":"additional","affiliation":[{"name":"Hematology Unit Department of Oncology and Hematology A.O.U of Modena, Policlinico  Modena Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicol\u00f2","family":"Mesini","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences Section of Hematology University of Modena and Reggio Emilia  Modena Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulia","family":"Debbia","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences Section of Hematology University of Modena 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