{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T10:40:02Z","timestamp":1781606402166,"version":"3.54.5"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,6,4]],"date-time":"2023-06-04T00:00:00Z","timestamp":1685836800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFC2500302 & 2017YFA0104500"],"award-info":[{"award-number":["2021YFC2500302 & 2017YFA0104500"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81621001"],"award-info":[{"award-number":["81621001"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Hematological Oncology"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    According to the 2022 European LeukemiaNet, all acute myeloid leukemia (AML) cases with\n                    <jats:italic>FLT3<\/jats:italic>\n                    \u2010ITD mutations are now categorized as intermediate risk irrespective of the\n                    <jats:italic>FLT3<\/jats:italic>\n                    \u2010ITD allelic ratio or concurrent presence of\n                    <jats:italic>NPM1<\/jats:italic>\n                    mutation. However, whether other next\u2010generation sequencing (NGS) comutation genes can add layers to\n                    <jats:italic>FLT3<\/jats:italic>\n                    \u2010ITD and whether the poor outcomes of\n                    <jats:italic>FLT3<\/jats:italic>\n                    \u2010ITD comutations can be overcome by haploidentical hematopoietic stem cell transplantation (haplo\u2010HSCT) are unclear. This study aimed to investigate which comutations based on NGS at diagnosis affect the clinical prognosis of de novo AML patients with\n                    <jats:italic>FLT3<\/jats:italic>\n                    \u2010ITD mutations and the effect of haplo\u2010HSCT on comutations. We analyzed 95 de novo AML patients with\n                    <jats:italic>FLT3<\/jats:italic>\n                    \u2010ITD mutations from January 2018 to August 2021 based on the NGS 99\u2010gene platform. Forty\u2010one other types of molecular mutations were detected. The most common cooccurring mutations were\n                    <jats:italic>NPM1<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a043, 45.3%) and\n                    <jats:italic>DNMT3A<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a021, 22.1%).\n                    <jats:italic>NPM1<\/jats:italic>\n                    mutation did not affect the clinical outcomes. Acute myeloid leukemia patients with\n                    <jats:italic>FLT3<\/jats:italic>\n                    \u2010ITD and\n                    <jats:italic>DNMT3A<\/jats:italic>\n                    comutations had significantly worse 3\u2010year Disease\u2010free survival (DFS) (49.5% vs. 69.3%,\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0=\u00a00.01) and Overall survival (OS) rates (61.1% vs. 69.8%,\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0=\u00a00.54) than those without\n                    <jats:italic>DNMT3A<\/jats:italic>\n                    mutations, and survival was significantly more favorable after haplo\u2010HSCT than that after chemotherapy (3\u2010year DFS, 85.7% vs. 30.8%,\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0=\u00a00.006; 3\u2010year OS, 85.7% vs. 43.1%,\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0=\u00a00.08). In multivariate analysis,\n                    <jats:italic>DNMT3A<\/jats:italic>\n                    mutation was a risk factor for DFS, while haplo\u2010HSCT was a protective factor. In conclusion,\n                    <jats:italic>DNMT3A<\/jats:italic>\n                    mutation might be a poor prognostic factor in adult AML patients with\n                    <jats:italic>FLT3<\/jats:italic>\n                    \u2010ITD mutations, and haplo\u2010HSCT could overcome the poor prognosis of\n                    <jats:italic>DNMT3A<\/jats:italic>\n                    comutation.\n                  <\/jats:p>","DOI":"10.1002\/hon.3186","type":"journal-article","created":{"date-parts":[[2023,6,6]],"date-time":"2023-06-06T22:35:14Z","timestamp":1686090914000},"page":"733-742","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The effect of haploidentical hematopoietic stem cell transplantation on comutations based on next\u2010generation sequencing in adult acute myeloid leukemia patients with the\n                    <i>FLT3<\/i>\n                    \u2010ITD mutation"],"prefix":"10.1002","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0460-9887","authenticated-orcid":false,"given":"Feifei","family":"Tang","sequence":"first","affiliation":[{"name":"Peking University People's Hospital Peking University Institute of Hematology National Clinical Research Center for Hematologic Disease Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaosu","family":"Zhao","sequence":"additional","affiliation":[{"name":"Peking University People's Hospital Peking University Institute of Hematology National Clinical Research Center for Hematologic Disease Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guorui","family":"Ruan","sequence":"additional","affiliation":[{"name":"Peking University People's Hospital Peking University Institute of Hematology National Clinical Research Center for Hematologic Disease Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Jiang","sequence":"additional","affiliation":[{"name":"Peking University People's Hospital Peking University Institute of Hematology National Clinical Research Center for Hematologic Disease Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Jiang","sequence":"additional","affiliation":[{"name":"Peking University People's Hospital Peking University Institute of Hematology National Clinical Research Center for Hematologic Disease Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lanping","family":"Xu","sequence":"additional","affiliation":[{"name":"Peking University People's Hospital Peking University Institute of Hematology National Clinical Research Center for Hematologic Disease Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Wang","sequence":"additional","affiliation":[{"name":"Peking University People's Hospital Peking University Institute of Hematology National Clinical Research Center for Hematologic Disease Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Peking University People's Hospital Peking University Institute of Hematology National Clinical Research Center for Hematologic Disease Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaiyan","family":"Liu","sequence":"additional","affiliation":[{"name":"Peking University People's Hospital Peking University Institute of Hematology National Clinical Research Center for Hematologic Disease Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2145-6643","authenticated-orcid":false,"given":"Xiaojun","family":"Huang","sequence":"additional","affiliation":[{"name":"Peking University People's Hospital Peking University Institute of Hematology National Clinical Research Center for Hematologic Disease Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation  Beijing China"},{"name":"Peking\u2010Tsinghua Center for Life Sciences  Beijing 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