{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T18:50:27Z","timestamp":1789498227596,"version":"build-2803163510"},"reference-count":17,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,1,5]],"date-time":"2024-01-05T00:00:00Z","timestamp":1704412800000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Hematological Oncology"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Flow cytometry (FCM) has become a method of choice for immunologic characterization of chronic lymphoproliferative disease (CLPD). To reduce the potential subjectivities of FCM data interpretation, we developed a machine learning random forest algorithm (RF) allowing unsupervised analysis. This assay relies on 16 parameters obtained from our FCM screening panel, routinely used in the exploration of peripheral blood (PB) samples (mean fluorescence intensity values (MFI) of CD19, CD45, CD5, CD20, CD200, CD23, HLA\u2010DR, CD10 in CD19\u2010gated B cells, ratio of kappa\/Lambda, and different ratios of MFI B\u2010cells\/T\u2010cells [CD20, CD200, CD23]). The RF algorithm was trained and validated on a large cohort of more than 300 annotated different CLPD cases (chronic B\u2010cell leukemia, mantle cell lymphoma, marginal zone lymphoma, follicular lymphoma, splenic red pulp lymphoma, hairy cell leukemia) and non\u2010tumoral selected from PB samples. The RF algorithm was able to differentiate tumoral from non\u2010tumoral B\u2010cells in all cases and to propose a correct CLPD classification in more than 90% of cases. In conclusion the RF algorithm could be proposed as an interesting help to FCM data interpretation allowing a first B\u2010cells CLPD diagnostic hypothesis and\/or to guide the management of complementary analysis (additional immunologic markers and genetic).<\/jats:p>","DOI":"10.1002\/hon.3245","type":"journal-article","created":{"date-parts":[[2024,1,5]],"date-time":"2024-01-05T05:15:17Z","timestamp":1704431717000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Diagnosis of chronic B\u2010cell lymphoproliferative disease in peripheral blood\u00a0=\u00a0how machine learning may help to the interpretation of flow cytometry data"],"prefix":"10.1002","volume":"42","author":[{"given":"Zofia","family":"Gross","sequence":"first","affiliation":[{"name":"Service clinique d'h\u00e9matologie Groupement Hospitalier Lyon\u2010Sud\/Hospices Civils de Lyon  Pierre\u2010B\u00e9nite France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Veyrat\u2010Masson","sequence":"additional","affiliation":[{"name":"Laboratoire d'h\u00e9matologie CHU ESTAING  Clermont Ferrand France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e9atrice","family":"Grange","sequence":"additional","affiliation":[{"name":"Laboratoire d'h\u00e9matologie sp\u00e9cialis\u00e9e Groupement Hospitalier Lyon\u2010Sud\/Hospices Civils de Lyon  Pierre\u2010B\u00e9nite France"},{"name":"Universit\u00e9 Claude Bernard Lyon 1 Centre International de Recherche en Infectiologie (CIRI) INSERM U1111 \u2010 CNRS UMR5308  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Huet","sequence":"additional","affiliation":[{"name":"Laboratoire d'h\u00e9matologie sp\u00e9cialis\u00e9e Groupement Hospitalier Lyon\u2010Sud\/Hospices Civils de Lyon  Pierre\u2010B\u00e9nite France"},{"name":"Universit\u00e9 Claude Bernard Lyon 1 Centre International de Recherche en Infectiologie (CIRI) INSERM U1111 \u2010 CNRS UMR5308  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aur\u00e9lie","family":"Verney","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Claude Bernard Lyon 1 Centre International de Recherche en Infectiologie (CIRI) INSERM U1111 \u2010 CNRS UMR5308  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Traverse\u2010Glehen","sequence":"additional","affiliation":[{"name":"Service d'anatomie\u2010pathologique Groupement Hospitalier Lyon\u2010Sud\/Hospices Civils de Lyon  Pierre\u2010B\u00e9nite France"},{"name":"INSERM U1245 Centre Henri Becquerel UNIROUEN University of Normandie  Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Ruminy","sequence":"additional","affiliation":[{"name":"INSERM U1245 Centre Henri Becquerel UNIROUEN University of Normandie  Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1357-229X","authenticated-orcid":false,"given":"Lucile","family":"Baseggio","sequence":"additional","affiliation":[{"name":"Laboratoire d'h\u00e9matologie sp\u00e9cialis\u00e9e Groupement Hospitalier Lyon\u2010Sud\/Hospices Civils de Lyon  Pierre\u2010B\u00e9nite France"},{"name":"Universit\u00e9 Claude Bernard Lyon 1 Centre International de Recherche en Infectiologie (CIRI) INSERM U1111 \u2010 CNRS UMR5308  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,1,5]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"SwerdlowS CampoE Lee HarrisN et\u00a0al.Mature B\u2010cell neoplasms. In:WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues (revised fourth edition) pp.215\u2010342.IARC;2017."},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2022015851"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375\u2010022\u201001613\u20101"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20362"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365\u20102257.2002.00135.x"},{"key":"e_1_2_8_7_1","first-page":"1640","article-title":"The immunological profile of B\u2010cell disorders and proposal of a scoring system for the diagnosis of CLL","volume":"8","author":"Matutes E","year":"1994","journal-title":"Leukemia"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1684\/abc.2023.1818"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2012.120"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1684\/abc.2021.1690"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41408\u2010020\u20100322\u20105"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102007\u201007\u2010098848"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/hon.957"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2013.3"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12824"},{"key":"e_1_2_8_16_1","first-page":"303","article-title":"The diagnostic value of CD123 in B\u2010cell disorders with hairy or villous lymphocytes","volume":"89","author":"Del Giudice I","year":"2004","journal-title":"Haematologica"},{"key":"e_1_2_8_17_1","first-page":"285","volume-title":"WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues (revised fourth edition","author":"Swerdlow S","year":"2017"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2009.011049"}],"container-title":["Hematological Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/hon.3245","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,26]],"date-time":"2024-03-26T11:08:41Z","timestamp":1711451321000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/hon.3245"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1]]},"references-count":17,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["10.1002\/hon.3245"],"URL":"https:\/\/doi.org\/10.1002\/hon.3245","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/HON.3245\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/HON.3245\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/HON.3245\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/HON.3245\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/HON.3245\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/HON.3245\/v2\/response1","asserted-by":"object"}]},"ISSN":["0278-0232","1099-1069"],"issn-type":[{"value":"0278-0232","type":"print"},{"value":"1099-1069","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1]]},"assertion":[{"value":"2023-08-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e3245"}}