{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T14:39:57Z","timestamp":1790260797061,"version":"4.1.0"},"reference-count":15,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,3,29]],"date-time":"2025-03-29T00:00:00Z","timestamp":1743206400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,3,29]],"date-time":"2025-03-29T00:00:00Z","timestamp":1743206400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Hematological Oncology"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Hematological malignancies often lack defined risk factors and present with non\u2010specific symptoms, underscoring the urgent need for simple and reliable detection methods. To address this challenge, Hirotsu et\u00a0al. innovated N\u2010NOSE, a novel, non\u2010invasive cancer screening test that utilizes the chemotaxis response of the nematode\n                    <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                    to detect tumor\u2010related odors in urine. In this clinical study, we assessed the performance of N\u2010NOSE in patients with various hematological malignancies at diagnosis and during treatment. Urine samples were collected from 30 healthy individuals and 89 patients, including those with leukemia (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a013), malignant lymphoma (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a053), multiple myeloma (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a015), primary AL amyloidosis (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a03), Waldenstr\u00f6m's macroglobulinemia (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a02), myelodysplastic syndrome (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a02), and blastic plasmacytoid dendritic cell neoplasm (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a01). Based on the optimal cut\u2010off values in detecting hematological malignancies, N\u2010NOSE demonstrated high positivity rates in treatment\u2010na\u00efve patients: leukemia and multiple myeloma were very high (over 90%), whereas malignant lymphoma was slightly lower than 80%. In the small subset of malignant lymphoma patients who tested N\u2010NOSE\u2010negative, confounding factors included steroid administration and hemodialysis. Importantly, no significant correlation emerged between N\u2010NOSE index values and baseline characteristics or comorbidities other than the presence of cancer. Moreover, in all 32 patients who achieved clinical response following chemotherapy, the N\u2010NOSE index declined, reflecting disease status. These findings highlight N\u2010NOSE's strong potential as a sensitive, non\u2010invasive screening tool for hematological malignancies\u2014particularly multiple myeloma\u2014and support its use in initial detection and monitoring of therapeutic response.\n                  <\/jats:p>","DOI":"10.1002\/hon.70062","type":"journal-article","created":{"date-parts":[[2025,3,29]],"date-time":"2025-03-29T15:39:42Z","timestamp":1743262782000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Detection of Hematological Malignancies Using N\u2010NOSE (Nematode\u2010NOSE)"],"prefix":"10.1002","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5465-2758","authenticated-orcid":false,"given":"Shingen","family":"Nakamura","sequence":"first","affiliation":[{"name":"Department of Community Medicine and Medical Science Tokushima University Graduate School of Biomedical Sciences  Tokushima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideyuki","family":"Hatakeyama","sequence":"additional","affiliation":[{"name":"Hirotsu Bio Science Inc.  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumiko","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Department of Hematology, Endocrinology and Metabolism Tokushima University Graduate School of Biomedical Sciences  Tokushima Japan"},{"name":"Department of Clinical Research National Hospital Organization Shikoku Medical Center for Children and Adults  Kagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9735-3798","authenticated-orcid":false,"given":"Umbhorn","family":"Ungkulpasvich","sequence":"additional","affiliation":[{"name":"Hirotsu Bio Science Inc.  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takaaki","family":"Hirotsu","sequence":"additional","affiliation":[{"name":"Hirotsu Bio Science Inc.  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"di Luccio","sequence":"additional","affiliation":[{"name":"Hirotsu Bio Science Inc.  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahiro","family":"Abe","sequence":"additional","affiliation":[{"name":"Department of Hematology Kawashima Hospital  Tokushima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,29]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885\u2010015\u20101857\u2010x"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0165\u20104608(98)00064\u20108"},{"key":"e_1_2_11_4_1","unstructured":"National Cancer Center Japan Cancer Registry and Statistics accessed December 10 2024 https:\/\/ganjoho.jp\/reg_stat\/statistics\/stat\/cancer\/."},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines12112546"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092\u20108674(93)80053\u2010h"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0092\u20108674(00)81068\u20105"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1895\/wormbook.1.66.1"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0118699"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrep.2024.101778"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1200\/jco.2013.54.8800"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1470\u20102045(16)30206\u20106"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102016\u201008\u2010733196"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms1750"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers15153870"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/93.14.1054"}],"container-title":["Hematological Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/hon.70062","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/hon.70062","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/hon.70062","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T09:26:06Z","timestamp":1772961966000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/hon.70062"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,29]]},"references-count":15,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["10.1002\/hon.70062"],"URL":"https:\/\/doi.org\/10.1002\/hon.70062","archive":["Portico"],"relation":{},"ISSN":["0278-0232","1099-1069"],"issn-type":[{"value":"0278-0232","type":"print"},{"value":"1099-1069","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,29]]},"assertion":[{"value":"2025-01-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70062"}}