{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T16:19:11Z","timestamp":1788279551351,"version":"build-2803163510"},"reference-count":21,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T00:00:00Z","timestamp":1780704000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T00:00:00Z","timestamp":1780704000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Hematological Oncology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Chronic lymphocytic leukemia (CLL) is a clinically and molecularly heterogeneous disease. PKMYT1, a G2\/M cell cycle kinase, has been implicated in tumor progression in several cancers, but its role in CLL remains unclear. We evaluated\n                    <jats:italic>PKMYT1<\/jats:italic>\n                    expression in primary CLL samples and analyzed associations with cytogenetic features and clinical parameters.\n                    <jats:italic>PKMYT1<\/jats:italic>\n                    expression was heterogeneous and correlated with adverse features, including complex karyotype and elevated leukocyte counts. Comparative transcriptomic analyses between high\u2010 and low\u2010expression groups revealed enrichment of pathways related to chromatin remodeling, DNA repair, and mitotic regulation. In MEC1 cells, pharmacological PKMYT1 inhibition significantly reduced cancer cell viability. PCR analysis showed upregulation of\n                    <jats:italic>TP53, CASP3, BAK, GSDMD, BCL2, CASP1, IL1\u03b2, RIPK1, and RIPK3<\/jats:italic>\n                    , indicating activation of apoptotic, inflammasome\u2010associated, and necroptotic pathways. Collectively, these findings demonstrate that\n                    <jats:italic>PKMYT1<\/jats:italic>\n                    is heterogeneously expressed in CLL, associated with adverse cytogenetic and clinical features, and critical for cell survival, highlighting its potential as a therapeutic target.\n                  <\/jats:p>","DOI":"10.1002\/hon.70208","type":"journal-article","created":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T10:54:17Z","timestamp":1780743257000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrative Transcriptomic and Functional Analysis of PKMYT1 Reveals a Potential Therapeutic Target in Chronic Lymphocytic Leukemia"],"prefix":"10.1002","volume":"44","author":[{"given":"Elizabete Cristina Iseke","family":"Bispo","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Hematologia e C\u00e9lulas\u2010Tronco Faculdade de Ci\u00eancias da Sa\u00fade Universidade de Bras\u00edlia  Bras\u00edlia Distrito Federal Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00e1udia de Souza Lima","family":"Pontes","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Hematologia e C\u00e9lulas\u2010Tronco Faculdade de Ci\u00eancias da Sa\u00fade Universidade de Bras\u00edlia  Bras\u00edlia Distrito Federal Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer Martins","family":"do Nascimento","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Hematologia e C\u00e9lulas\u2010Tronco Faculdade de Ci\u00eancias da Sa\u00fade Universidade de Bras\u00edlia  Bras\u00edlia Distrito Federal Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e1bio Wilson de Lima","family":"Alves","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Hematologia e C\u00e9lulas\u2010Tronco Faculdade de Ci\u00eancias da Sa\u00fade Universidade de Bras\u00edlia  Bras\u00edlia Distrito Federal Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana Lott","family":"de Carvalho","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Bioci\u00eancias Faculdade de Medicina Universidade de Bras\u00edlia  Bras\u00edlia Distrito Federal Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ant\u00f4nio Roberto","family":"Lucena\u2010Araujo","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Hematologia Centro de Bioci\u00eancias Universidade Federal de Pernambuco  Recife Pernambuco Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4200-0821","authenticated-orcid":false,"given":"Felipe","family":"Saldanha\u2010Araujo","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Hematologia e C\u00e9lulas\u2010Tronco Faculdade de Ci\u00eancias da Sa\u00fade Universidade de Bras\u00edlia  Bras\u00edlia Distrito Federal Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,6]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571\u2010019\u20100239\u20108"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.19986"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.26146"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2014.296"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0193098"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045\u2010020\u201000959\u20102"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/em.22128"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv1101"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41588\u2010022\u201001140\u2010w"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv1070"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.27"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.beha.2016.08.002"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2015.00207"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3109\/10428194.2013.796058"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s44321\u2010024\u201000060\u2010y"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010025\u201002250\u20107"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2015.11.021"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010025\u201058183\u2010w"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419\u2010025\u201007835\u20102"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/hem3.70322"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2025029905"}],"container-title":["Hematological 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