{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T16:19:14Z","timestamp":1788279554968,"version":"build-2803163510"},"reference-count":41,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T00:00:00Z","timestamp":1781740800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T00:00:00Z","timestamp":1781740800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Hematological Oncology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>RUNX1<\/jats:italic>\n                    is one of the most commonly mutated genes in myelodysplastic neoplasms (MDS) and essential for hematopoiesis. However, clinical phenotype features of\n                    <jats:italic>RUNX1<\/jats:italic>\n                    mutated patients and the interaction between\n                    <jats:italic>RUNX1<\/jats:italic>\n                    and other genes mutations remains incompletely explored. We enrolled 1473 consecutive adult patients with primary MDS to investigate the laboratory, genomic characteristics, and survival of\n                    <jats:italic>RUNX1<\/jats:italic>\n                    \u2010mutated MDS subjects. We found that\n                    <jats:italic>RUNX1<\/jats:italic>\n                    mutations are associated with older age (median, 59 vs. 56\u00a0years,\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0=\u00a00.007), lower platelet counts (median, 50\u00a0\u00d7\u00a010\n                    <jats:sup>9<\/jats:sup>\n                    \/L vs. 66\u00a0\u00d7\u00a010\n                    <jats:sup>9<\/jats:sup>\n                    \/L;\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.001), higher bone marrow blasts (median, 6.0% vs. 2.5%,\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.001) and a higher proportion of micro\u2010megakaryocytes (median, 26.0% vs. 18.7%,\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.001). Additionally, our data showed a correlation between the variant allele frequency (VAF) of\n                    <jats:italic>RUNX1<\/jats:italic>\n                    mutations and proportion of micro\u2010megakaryocytes (\n                    <jats:italic>r<\/jats:italic>\n                    \u00a0=\u00a00.242,\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0=\u00a00.006), suggesting\n                    <jats:italic>RUNX1<\/jats:italic>\n                    mutations burden was associated with dysmegakaryopoiesis. Mutations in\n                    <jats:italic>ASXL1<\/jats:italic>\n                    ,\n                    <jats:italic>SRSF2<\/jats:italic>\n                    ,\n                    <jats:italic>EZH2<\/jats:italic>\n                    and\n                    <jats:italic>NRAS<\/jats:italic>\n                    were significantly more frequent in\n                    <jats:italic>RUNX1<\/jats:italic>\n                    \u2010mutated patients compared with those without\n                    <jats:italic>RUNX1<\/jats:italic>\n                    mutations (adjusted\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.05).\n                    <jats:italic>RUNX1<\/jats:italic>\n                    \u2010mutated patients exhibited poorer overall survival (median OS 18\u00a0months vs. 51\u00a0month,\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.001), while\n                    <jats:italic>U2AF1<\/jats:italic>\n                    co\u2010mutations were associated with a relatively better prognosis (median OS 34\u00a0months vs. 17\u00a0months,\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0=\u00a00.003), indicating a potential modifying effect of\n                    <jats:italic>U2AF1<\/jats:italic>\n                    on the outcome of\n                    <jats:italic>RUNX1<\/jats:italic>\n                    \u2010mutated patients.\n                  <\/jats:p>","DOI":"10.1002\/hon.70212","type":"journal-article","created":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T16:50:07Z","timestamp":1781801407000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Impact of\n                    <i>U2AF1<\/i>\n                    Pathogenic Variants on Prognosis of Myelodysplastic Neoplasms With\n                    <i>RUNX1<\/i>\n                    Mutation"],"prefix":"10.1002","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-1044-5897","authenticated-orcid":false,"given":"Linlin","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Experimental Hematology National Clinical Research Center for Blood Diseases Haihe Laboratory of Cell Ecosystem Institute of Hematology &amp; Blood Diseases Hospital Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Tianjin China"},{"name":"Tianjin Institutes of Health Science  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8291-9883","authenticated-orcid":false,"given":"Bing","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology National Clinical Research Center for Blood Diseases Haihe Laboratory of Cell Ecosystem Institute of Hematology &amp; Blood Diseases Hospital Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Tianjin China"},{"name":"Tianjin Institutes of Health Science  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiejun","family":"Qin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology National Clinical Research Center for Blood Diseases Haihe Laboratory of Cell Ecosystem Institute of Hematology &amp; Blood Diseases Hospital Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Tianjin China"},{"name":"Tianjin Institutes of Health Science  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zefeng","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology National Clinical Research Center for Blood Diseases Haihe Laboratory of Cell Ecosystem Institute of Hematology &amp; Blood Diseases Hospital Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Tianjin China"},{"name":"Tianjin Institutes of Health Science  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiqiang","family":"Qu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology National Clinical Research Center for Blood Diseases Haihe Laboratory of Cell Ecosystem Institute of Hematology &amp; Blood Diseases Hospital Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Tianjin China"},{"name":"Tianjin Institutes of Health Science  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijuan","family":"Pan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology National Clinical Research Center for Blood Diseases Haihe Laboratory of Cell Ecosystem Institute of Hematology &amp; Blood Diseases Hospital Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Tianjin China"},{"name":"Tianjin Institutes of Health Science  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingyan","family":"Gao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology National Clinical Research Center for Blood Diseases Haihe Laboratory of Cell Ecosystem Institute of Hematology &amp; Blood Diseases Hospital Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Tianjin China"},{"name":"Tianjin Institutes of Health Science  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Jiao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology National Clinical Research Center for Blood Diseases Haihe Laboratory of Cell Ecosystem Institute of Hematology &amp; Blood Diseases Hospital Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Tianjin China"},{"name":"Tianjin Institutes of Health Science  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zefei","family":"Bao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology National Clinical Research Center for Blood Diseases Haihe Laboratory of Cell Ecosystem Institute of Hematology &amp; Blood Diseases Hospital Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Tianjin China"},{"name":"Tianjin Institutes of Health Science  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ningning","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology National Clinical Research Center for Blood Diseases Haihe Laboratory of Cell Ecosystem Institute of Hematology &amp; Blood Diseases Hospital Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Tianjin China"},{"name":"Tianjin Institutes of Health Science  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujiao","family":"Jia","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology National Clinical Research Center for Blood Diseases Haihe Laboratory of Cell Ecosystem Institute of Hematology &amp; Blood Diseases Hospital Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Tianjin China"},{"name":"Tianjin Institutes of Health Science  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengwen","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology National Clinical Research Center for Blood Diseases Haihe Laboratory of Cell Ecosystem Institute of Hematology &amp; Blood Diseases Hospital Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Tianjin China"},{"name":"Tianjin Institutes of Health Science  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1099-4489","authenticated-orcid":false,"given":"Zhijian","family":"Xiao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology National Clinical Research Center for Blood Diseases Haihe Laboratory of Cell Ecosystem Institute of Hematology &amp; Blood Diseases Hospital Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Tianjin China"},{"name":"Tianjin Institutes of Health Science  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,18]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2022015850"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102141.2007.06811.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u20100609.2004.00363.x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102003\u201009\u20103074"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2013.336"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/EVIDoa2200008"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375\u2010022\u201001718\u20107"},{"issue":"7","key":"e_1_2_9_9_1","first-page":"1789s","article-title":"Core\u2010Binding Factor: A Central Player in Hematopoiesis and Leukemia","volume":"59","author":"Speck N. 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