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We characterized usage, mutation frequency and mutation pattern of immunoglobulin variable (IGV) gene rearrangements in 50 PTLD (polymorphic PTLD, <jats:italic>n<\/jats:italic>=10; diffuse large B\u2010cell lymphoma, <jats:italic>n<\/jats:italic>=35; and Burkitt\/Burkitt\u2010like lymphoma, <jats:italic>n<\/jats:italic>=5). Among PTLD yielding clonal IGV amplimers, a functional IGV heavy chain (IGHV) rearrangement was found in 40\/50 (80.0%) cases, whereas a potentially functional IGV light chain rearrangement was identified in 36\/46 (78.3%) PTLD. By combining IGHV and IGV light chain rearrangements, 10\/50 (20.0%) PTLD carried crippling mutations, precluding expression of a functional B\u2010cell receptor (BCR). Immunohistochemistry showed detectable expression of IG light chains in only 18\/43 (41.9%) PTLD. Failure to detect a functional IGV rearrangement associated with lack of IGV expression. Our data suggest that a large fraction of PTLD arise from germinal centre (GC)\u2010experienced B\u2010cells that display impaired BCR. Since a functional BCR is required for normal B\u2010cell survival during GC transit, PTLD development may implicate rescue from apoptosis and expansion of B\u2010cells that have failed the GC reaction. The high frequency of IGV loci inactivation appears to be a peculiar feature of PTLD among immunodeficiency\u2010associated lymphoproliferations. Copyright \u00a9 2006 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/hon.791","type":"journal-article","created":{"date-parts":[[2006,8,9]],"date-time":"2006-08-09T10:29:08Z","timestamp":1155119348000},"page":"212-219","source":"Crossref","is-referenced-by-count":19,"title":["Analysis of immunoglobulin heavy and light chain variable genes in post\u2010transplant lymphoproliferative disorders"],"prefix":"10.1002","volume":"24","author":[{"given":"Daniela","family":"Capello","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michaela","family":"Cerri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuliana","family":"Muti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Lucioni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierluigi","family":"Oreste","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annunziata","family":"Gloghini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Berra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Deambrogi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Franceschetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davide","family":"Rossi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oscar","family":"Alabiso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrica","family":"Morra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Rambaldi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonino","family":"Carbone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Paulli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianluca","family":"Gaidano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,8,9]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"106","article-title":"Malignant lymphoma in transplantation patients","volume":"1","author":"Penn I","year":"1969","journal-title":"Transplant Proc"},{"key":"e_1_2_1_3_2","volume-title":"Post\u2010Transplant Lymphoproliferative Disorders","author":"Harris N","year":"2001"},{"key":"e_1_2_1_4_2","volume-title":"Pathology and Genetics. 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