{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T03:18:55Z","timestamp":1789096735555,"version":"build-2803163510"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,3,8]],"date-time":"2009-03-08T00:00:00Z","timestamp":1236470400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hematological Oncology"],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Acquired mutations in the juxtamembrane region of <jats:italic>MPL<\/jats:italic> (W515L or W515K), the receptor for thrombopoietin, have been reported in patients with primary essential thrombocythemia (ET) or primary myelofibrosis (PMF). The mutations were detected by the newly developed real\u2010time quantitative PCR (RQ\u2010PCR) with TaqMan MGB probes and followed by the sequencing analysis. DNA samples were from 343 Chinese adults with JAK2V617F mutation\u2010negative chronic myeloproliferative disorders (cMPDs). Reference curves were obtained using cloned fragments of <jats:italic>MPL<\/jats:italic> containing either the wild\u2010type or <jats:italic>MPL<\/jats:italic> W515L or <jats:italic>MPL<\/jats:italic> W515K mutated sequence; the predicted sensitivity level was at least 0.5%(0.1\u20130.5%) for <jats:italic>MPL<\/jats:italic> W515L and 0.5%(0.2\u20130.5%) for <jats:italic>MPL<\/jats:italic> W515K mutant allele in a wild\u2010type background. The detection rates of <jats:italic>MPL<\/jats:italic> W515 mutations were 3.5% in 199 ET patients (7\/199), 12.5% in 24 PMF patients (3\/24) and 5.6% in 36 cMPD\u2010unclassed patients (2\/36), respectively. No <jats:italic>MPL<\/jats:italic> W515 mutations were detected in 32 polycythemia vera (PV) patients, 40 chronic myeloid leukaemia (CML) patients, 12 hypereosinophilic syndrome (HES) patients and 29 normal volunteers. The mean calculated burden of <jats:italic>MPL<\/jats:italic> mutant alleles using RQ\u2010PCR for <jats:italic>MPL<\/jats:italic> W515L\/K was 24.88\u2009\u00b1\u200914.80% (range, 1.10\u201356.32%). <jats:italic>MPL<\/jats:italic> W515L\/K patients presented lower haemoglobin levels, compared with the patients with JAK2V617F mutation\u2010positive cMPDs (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01). The results demonstrated that RQ\u2010PCR was a reliable and sensitive method for large\u2010scale screening of the <jats:italic>MPL<\/jats:italic> W515L\/K mutation in patients suspected to have a cMPD. It can also provide a quantitative estimate of mutant allele burden that might be useful for both patient prognosis and monitoring response to therapy. Copyright \u00a9 2009 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/hon.899","type":"journal-article","created":{"date-parts":[[2009,3,9]],"date-time":"2009-03-09T01:14:08Z","timestamp":1236561248000},"page":"33-39","source":"Crossref","is-referenced-by-count":13,"title":["<i>MPL<\/i> W515L\/K mutations in 343 Chinese adults with JAK2V617F mutation\u2010negative chronic myeloproliferative disorders detected by a newly developed RQ\u2010PCR based on TaqMan MGB probes"],"prefix":"10.1002","volume":"28","author":[{"given":"Guo\u2010Rui","family":"Ruan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling\u2010Di","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji\u2010Hong","family":"Niu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin\u2010Lan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya\u2010Zhen","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan\u2010Rong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010Jun","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan\u2010Shan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,3,8]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-04-1199"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-04-083501"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature03546"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)71142-9"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2005.03.023"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa051113"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-12-064287"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403902"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)67785-9"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2005.05764.x"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.11653"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-08-3395"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2005.05776.x"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-07-099184"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2405097"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0030270"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-04-018879"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2007.06565.x"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2006.06348.x"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-06-2600"},{"issue":"34","key":"e_1_2_1_22_2","first-page":"2401","article-title":"Detection 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