{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,29]],"date-time":"2025-03-29T16:24:32Z","timestamp":1743265472528},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,3,7]],"date-time":"2011-03-07T00:00:00Z","timestamp":1299456000000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hematological Oncology"],"published-print":{"date-parts":[[2011,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Analyses of the tumour immunoglobulin (Ig) gene (<jats:italic>IG<\/jats:italic>) heavy (<jats:italic>H<\/jats:italic>) and light chains show heterogeneity of mutational status, but reveal common features of ongoing IGH isotype\u2010switching with multiple IGH isotype expression and preference of <jats:italic>IG<\/jats:italic> lambda (<jats:italic>IGL<\/jats:italic>) light chain with selective use of <jats:italic>IGLJ3<\/jats:italic>. Phenotypic and immunogenetic analyses were performed in a series of 105 HCL patients to estimate prevalence of multiple <jats:italic>IG<\/jats:italic> light chain expression by the tumour cells. By phenotype, 3\/105 HCL (2.9%) expressed double tumour\u2010related Ig kappa (<jats:italic>K<\/jats:italic>) and L light chain proteins. By immunogenetic analysis, functional mutated double <jats:italic>IGK<\/jats:italic><jats:sup><jats:italic>I<\/jats:italic><\/jats:sup><jats:italic>\/IGK<\/jats:italic><jats:sup><jats:italic>II<\/jats:italic><\/jats:sup>, <jats:italic>IGK<\/jats:italic><jats:sup><jats:italic>I<\/jats:italic><\/jats:sup><jats:italic>\/IGL<\/jats:italic><jats:sup><jats:italic>I<\/jats:italic><\/jats:sup> and <jats:italic>IGL<\/jats:italic><jats:sup><jats:italic>I<\/jats:italic><\/jats:sup><jats:italic>\/IGL<\/jats:italic><jats:sup><jats:italic>II<\/jats:italic><\/jats:sup> transcripts were cloned and sequenced in 3\/71 (4.2%) HCL. These latter three HCL expressed multiple <jats:italic>IGH<\/jats:italic> isotypes with mutated <jats:italic>IGHVDJ<\/jats:italic> rearrangements at the time of <jats:italic>AID<\/jats:italic> transcript expression. Most interestingly, the three cases had reinduced <jats:italic>RAG1<\/jats:italic> transcript. In the double <jats:italic>IGL<\/jats:italic> expresser, single\u2010cell analysis documented co\u2010expression of the tumour\u2010related <jats:italic>IGLs<\/jats:italic> in 5\/6 cells (83%). In the <jats:italic>IGK\/IGL<\/jats:italic> co\u2010expresser, evidence of surface IgK\/IgL isotype proteins confirmed functionality of the tumour\u2010derived transcripts. The evidence of double light chain expression in single HCs and the new observation of <jats:italic>RAG<\/jats:italic> re\u2010induction suggest ongoing selective influences on the BCR that may promote or maintain the HCL clone in the periphery. Copyright \u00a9 2010 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/hon.952","type":"journal-article","created":{"date-parts":[[2010,7,23]],"date-time":"2010-07-23T09:01:27Z","timestamp":1279875687000},"page":"31-37","source":"Crossref","is-referenced-by-count":6,"title":["Lack of allelic exclusion by secondary rearrangements of tumour B\u2010cell receptor light chains in hairy cell leukaemia"],"prefix":"10.1002","volume":"29","author":[{"given":"Elisa","family":"Sozzi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teresa","family":"Amato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Surinder 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