{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T17:57:50Z","timestamp":1769277470291,"version":"3.49.0"},"reference-count":41,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T00:00:00Z","timestamp":1700092800000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005117","name":"Birjand University of Medical Sciences","doi-asserted-by":"publisher","award":["456582"],"award-info":[{"award-number":["456582"]}],"id":[{"id":"10.13039\/501100005117","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Health Science Reports"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Aims<\/jats:title><jats:p>Gene polymorphisms are responsible for at least part of the variation in caries susceptibility despite similar environmental factors. Genes involved in enamel formation like matrix metalloproteinase\u201013 (<jats:italic>MMP\u201013<\/jats:italic>) may participate in caries process. The aim was to investigate the association between <jats:italic>MMP\u201013<\/jats:italic> rs478927 polymorphism and caries susceptibility in 6\u2010years\u2010old children from Birjand, Iran.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Six\u2010years old children from Birjand, Iran, participated in this study. The total decayed, missing, and filled teeth were calculated and defined as caries index (CI). Based on this CI, two groups of high\u2010caries (case) and low\u2010caries (control) were taken into account. Saliva samples were collected and DNA was extracted. The allele and genotypes of <jats:italic>MMP\u201013<\/jats:italic> rs478927 polymorphism were determined by tetra\u2010primer amplification refractory mutation system\u2010polymerase chain reaction (T\u2010ARMS\u2010PCR) method. <jats:italic>p<\/jats:italic> Value was significant if <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Three hundred sixty\u2010seven children consisted of 186 low\u2010caries children and 181 high\u2010caries children were included in this study. The mean CI was 6.02\u2009\u00b1\u20090.81. There was no significant association between high and low\u2010caries groups based on socioeconomic status, eating sweet snacks, parents' susceptibility to dental caries, duration of breastfeeding, and the brushing habit (<jats:italic>p<\/jats:italic>\u2009&gt;\u20090.05). There wasn't any significant association between genotype distribution of <jats:italic>MMP\u201013<\/jats:italic> rs478927 polymorphism and CI groups (<jats:italic>p<\/jats:italic>\u2009=\u20090.924). This polymorphism was associated with increased caries susceptibility under all genetic models but this effect was not significant (<jats:italic>p<\/jats:italic>\u2009&gt;\u20090.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The <jats:italic>MMP\u201013<\/jats:italic> rs478927 gene polymorphism was not significantly associated with dental caries susceptibility in Birjandi children with mixed dentition. It is recommended to conduct studies on children of different dentitions to better understand the role of this polymorphism on caries susceptibility in primary and permanent teeth of children.<\/jats:p><\/jats:sec>","DOI":"10.1002\/hsr2.1708","type":"journal-article","created":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T08:32:40Z","timestamp":1700209960000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The association between <i>MMP<\/i>\u221213 rs478927 gene polymorphism and dental caries susceptibility in children with mixed dentition from Birjand, Iran: A case\u2010control study"],"prefix":"10.1002","volume":"6","author":[{"given":"Ebrahim","family":"Miri\u2010Moghaddam","sequence":"first","affiliation":[{"name":"Department of Molecular Medicine, Cardiovascular Diseases Research Center, Faculty of Medicine Birjand University of Medical Sciences Birjand 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