{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T12:47:03Z","timestamp":1779108423377,"version":"3.51.4"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,5,9]],"date-time":"2018-05-09T00:00:00Z","timestamp":1525824000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["311309\/2012\u20107"],"award-info":[{"award-number":["311309\/2012\u20107"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004263","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Rio Grande do Sul","doi-asserted-by":"publisher","award":["2282\u20102551\/14\u20105"],"award-info":[{"award-number":["2282\u20102551\/14\u20105"]}],"id":[{"id":"10.13039\/501100004263","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004263","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Rio Grande do Sul","doi-asserted-by":"publisher","award":["2282\u20132551\/14\u20135"],"award-info":[{"award-number":["2282\u20132551\/14\u20135"]}],"id":[{"id":"10.13039\/501100004263","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Health Science Reports"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>Influenza A virus (IAV) can cause severe acute respiratory infection (SARI), and disease outcome may be associated with changes in the microbiome of the nasopharynx. This is a pilot study to characterize the microbiome of the nasopharynx in patients hospitalized with SARI, infected and not infected by IAV.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and Results<\/jats:title><jats:p>Using target sequencing of the 16S rRNA gene, we assessed the bacterial community of nasopharyngeal aspirate samples and compared the microbiome of patients infected with IAV with the microbiome of patients who were negative for IAV. We observed differences in the relative abundance of Proteobacteria and Firmicutes between SARI patients, with <jats:italic>Streptococcus<\/jats:italic> being enriched and <jats:italic>Pseudomonas<\/jats:italic> underrepresented in IAV patients compared with patients who were not infected with IAV.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:italic>Pseudomonas<\/jats:italic> taxon seems to be in high frequency on the nasopharynx of SARI patients with non\u2010IAV infection and might present a negative association with <jats:italic>Streptococcus<\/jats:italic> taxon. Microbial profile appears to be different between SARI patients infected or not infected with IAV.<\/jats:p><\/jats:sec>","DOI":"10.1002\/hsr2.47","type":"journal-article","created":{"date-parts":[[2018,5,10]],"date-time":"2018-05-10T06:55:23Z","timestamp":1525935323000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Comparison of the nasopharynx microbiome between influenza and non\u2010influenza cases of severe acute respiratory infections: A pilot study"],"prefix":"10.1002","volume":"1","author":[{"given":"Luiz Gustavo dos Anjos","family":"Borges","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Patologia Universidade Federal de Ci\u00eancias da Sa\u00fade de Porto Alegre (UFCSPA)  Porto Alegre RS Brazil"},{"name":"Department of Microbiology Icahn School of Medicine at Mount Sinai  New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana","family":"Giongo","sequence":"additional","affiliation":[{"name":"Instituto do Petr\u00f3leo e dos Recursos Naturais (IPR) Pontif\u00edcia Universidade Cat\u00f3lica do Rio Grande do Sul (PUCRS)  Porto Alegre RS Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leandro de Mattos","family":"Pereira","sequence":"additional","affiliation":[{"name":"Faculdade de Bioci\u00eancias Pontif\u00edcia Universidade Cat\u00f3lica do Rio Grande do Sul (PUCRS)  Porto Alegre RS Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernanda J.","family":"Trindade","sequence":"additional","affiliation":[{"name":"Faculdade de Bioci\u00eancias Pontif\u00edcia Universidade Cat\u00f3lica do Rio Grande do Sul (PUCRS)  Porto Alegre RS Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatiana Sch\u00e4ffer","family":"Gregianini","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Central de Sa\u00fade P\u00fablica da Secretaria de Sa\u00fade do Estado do Rio Grande do Sul (LACEN\/SES\u2010RS)  Porto Alegre RS Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabr\u00edcio Souza","family":"Campos","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine and Agronomy University of Bras\u00edlia, Darcy Ribeiro University Campus, ICC  Asa Norte, CEP 70.910-970 Bras\u00edlia DF Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elodie","family":"Ghedin","sequence":"additional","affiliation":[{"name":"Center for Genomics and Systems Biology, Department of Biology New York University  New York NY USA"},{"name":"Department of Epidemiology, College of Global Public Health New York University  New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1462-5506","authenticated-orcid":false,"given":"Ana Beatriz Gorini","family":"da Veiga","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Patologia Universidade Federal de Ci\u00eancias da Sa\u00fade de Porto Alegre (UFCSPA)  Porto Alegre RS Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,5,9]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2010.05.009"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/aje\/kwv054"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2245"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2015.09.011"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11234"},{"key":"e_1_2_10_7_1","article-title":"The human salivary microbiome exhibits temporal stability in bacterial diversity","volume":"91","author":"Cameron SJS","year":"2015","journal-title":"FEMS Microbiol Ecol"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201210-1913OC"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-015-0074-9"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.000920"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003057"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-016-0182-1"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/irv.12080"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1019378108"},{"issue":"102","key":"e_1_2_10_15_1","first-page":"33","article-title":"Why is coinfection with influenza virus and bacteria so difficult to control?","volume":"19","author":"Cauley LS","year":"2015","journal-title":"Discov Med"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0017035"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2014.0294"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.12.29"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1086\/656333"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-015-0133-2"},{"key":"e_1_2_10_21_1","unstructured":"CDC.CDC protocol of realtime RT\u2010PCR for influenza A (H1N1). Vol 1.;2009.http:\/\/www.who.int\/csr\/resources\/publications\/swineflu\/realtimeptpcr\/en\/index.html"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2010.171"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12915-014-0087-z"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr026"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq461"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt1244"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.f.303"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03006-05"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/2047-217X-2-16"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1086\/591708"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005975"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1097\/PCC.0b013e3181ccedae"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0902772"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12054"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1136\/thoraxjnl-2011-201183"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2016.01.001"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1206552109"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00267-17"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pathmechdis.3.121806.154316"}],"container-title":["Health Science Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fhsr2.47","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/hsr2.47","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T20:40:29Z","timestamp":1695501629000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/hsr2.47"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,5,9]]},"references-count":38,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2018,6]]}},"alternative-id":["10.1002\/hsr2.47"],"URL":"https:\/\/doi.org\/10.1002\/hsr2.47","archive":["Portico"],"relation":{},"ISSN":["2398-8835","2398-8835"],"issn-type":[{"value":"2398-8835","type":"print"},{"value":"2398-8835","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,5,9]]},"assertion":[{"value":"2017-11-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-04-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-05-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e47"}}