{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T07:04:01Z","timestamp":1769151841255,"version":"3.49.0"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,1,20]],"date-time":"2022-01-20T00:00:00Z","timestamp":1642636800000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005378","name":"Hacettepe \u00dcniversitesi","doi-asserted-by":"publisher","award":["THD\u20102017\u201014742"],"award-info":[{"award-number":["THD\u20102017\u201014742"]}],"id":[{"id":"10.13039\/501100005378","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Health Science Reports"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Aims<\/jats:title><jats:p>Influenza virus is one of the leading infections causing death among human being. Despite known risks, primary immune deficiency due to Interferon Regulatory Factor\u20107 (<jats:styled-content style=\"fixed-case\">IRF7<\/jats:styled-content>) gene defect was reported as a possible cause of the risk factors for complicated influenza. We aimed to investigate the changes in peripheral T and B cell subsets in adult patients with severe seasonal influenza virus infection and the investigation of variants of <jats:styled-content style=\"fixed-case\"><jats:italic>IRF7<\/jats:italic><\/jats:styled-content> gene.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this study, 32 patients, hospitalized due to influenza infection\u2010related acute respiratory failure were included.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The median age of the patients was 76\u2009years (26\u201096), and 13\/32 (40.6%) were in the intensive care unit. Central memory Th, effector memory Th, <jats:styled-content style=\"fixed-case\">TEMRA<\/jats:styled-content> Th, cytotoxic T lymphocytes (<jats:styled-content style=\"fixed-case\">CTL<\/jats:styled-content>), central memory <jats:styled-content style=\"fixed-case\">CTL<\/jats:styled-content> of the patients were found to be increased, naive <jats:styled-content style=\"fixed-case\">CTL<\/jats:styled-content> were decreased. There was a significant increase in the percentage of effector memory Th, and a decrease in the percentage of naive <jats:styled-content style=\"fixed-case\">CTL<\/jats:styled-content> in patients \u226565\u2009years\u2010old compared to patients &lt;65\u2009years old (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.039, and <jats:italic>P<\/jats:italic>\u00a0=\u00a0.017, respectively). <jats:styled-content style=\"fixed-case\"><jats:italic>IRF7<\/jats:italic><\/jats:styled-content> gene analysis revealed two different nucleotide changes in three patients; c.535 A\u2009&gt;\u2009G; p.<jats:styled-content style=\"fixed-case\">Lys179Glu<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\"><jats:italic>K179E<\/jats:italic><\/jats:styled-content>) and <jats:styled-content style=\"fixed-case\">c584A<\/jats:styled-content>\u00a0&gt;\u2009T; p.<jats:styled-content style=\"fixed-case\">His195Leu<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\"><jats:italic>H195L<\/jats:italic><\/jats:styled-content>), located in the fourth exon of the <jats:styled-content style=\"fixed-case\"><jats:italic>IRF7<\/jats:italic><\/jats:styled-content> gene.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>The increases in central and effector memory Th, central memory CTL and decrease of naive CTLs may be secondary to the virus infection. K179E (rs1061502) and H195L (rs139709725) variants were not reported to be related with susceptibility to an infection yet. It is conceivable to investigate for novel variants in other genes related to antiviral immunity.<\/jats:p><\/jats:sec>","DOI":"10.1002\/hsr2.492","type":"journal-article","created":{"date-parts":[[2022,1,20]],"date-time":"2022-01-20T11:26:40Z","timestamp":1642678000000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of the peripheral blood T and B cell subsets and <scp>IRF<\/scp>\u20107 variants in adult patients with severe influenza virus infection"],"prefix":"10.1002","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1290-6905","authenticated-orcid":false,"given":"Nursel","family":"\u00c7al\u0131k Ba\u015faran","sequence":"first","affiliation":[{"name":"Faculty of Medicine, Department of Internal Medicine, General Internal Medicine Division Hacettepe University  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6972-1349","authenticated-orcid":false,"given":"\u00c7a\u011fman","family":"Tan","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, \u0130hsan Do\u011framac\u0131 Children's Hospital, Section of Pediatric Immunology Hacettepe University  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3494-8997","authenticated-orcid":false,"given":"Lale","family":"\u00d6z\u0131\u015f\u0131k","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Internal Medicine, General Internal Medicine Division Hacettepe University  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9053-2785","authenticated-orcid":false,"given":"Beg\u00fcm","family":"\u00d6zbek","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, \u0130hsan Do\u011framac\u0131 Children's Hospital, Section of Pediatric Immunology Hacettepe University  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7943-8715","authenticated-orcid":false,"given":"Ahmet \u00c7a\u011fkan","family":"\u0130nkaya","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Infectious Diseases and Clinical Microbiology Hacettepe University  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9424-8346","authenticated-orcid":false,"given":"\u015eehnaz","family":"Alp","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Infectious Diseases and Clinical Microbiology Hacettepe University  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2534-1333","authenticated-orcid":false,"given":"Ebru Orta\u00e7","family":"Ersoy","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Internal Medicine, Intensive Care Division Hacettepe University  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2213-4627","authenticated-orcid":false,"given":"Deniz \u00c7a\u011fda\u015f","family":"Ayvaz","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, \u0130hsan Do\u011framac\u0131 Children's Hospital, Section of Pediatric Immunology Hacettepe University  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u0130lhan","family":"Tezcan","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, \u0130hsan Do\u011framac\u0131 Children's Hospital, Section of Pediatric Immunology Hacettepe University  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,1,20]]},"reference":[{"key":"e_1_2_12_2_1","unstructured":"World Health Organization.Influenza (Seasonal).https:\/\/www.who.int\/en\/news-room\/fact-sheets\/detail\/influenza-(seasonal). 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