{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T17:25:49Z","timestamp":1783445149692,"version":"3.54.6"},"reference-count":50,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T00:00:00Z","timestamp":1727222400000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Health Science Reports"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Aims<\/jats:title><jats:p>The pathophysiology of haemophilic arthropathy (HA) is complex and largely undefined. Proteomic analyses provide insights into the intricate mechanisms of the HA.<\/jats:p><jats:p>Our study aimed to identify differentially expressed proteins in relation to the severity of HA, explore their pathophysiological roles, and evaluate their potential as HA biomarkers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Our cross\u2010sectional observational study encompassed 30 HA patients and 15 healthy subjects. Plasma samples were pooled into three groups of 15 samples from those with severe haemophilic arthropathy (sHA), mild haemophilic arthropathy (mHA) and healthy controls. Proteomic analysis was performed using liquid chromatography\u2010mass spectrometry. The severity of HA was assessed using the World Federation of Haemophilia Physical Examination Score and ultrasonography following the Haemophilia Early Arthropathy Detection with Ultrasound (HEAD\u2010US) guidelines.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 788 proteins were identified, with 97% of the uniquely identified proteins being expressed in all analysed groups. We identified several up and downregulated proteins across the groups that were mainly related to inflammatory and immunity\u2010modulating processes, as well as joint degeneration. We highlighted ten proteins relevant for the development of HA: cathepsin G, endoplasmic reticulum aminopeptidase 2, S100\u2010A9, insulin\u2010like growth factor I, apolipoprotein (a), osteopontin, pregnancy zone protein, cartilage oligomeric matrix protein, CD44, and cadherin\u2010related family member 2.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our analysis identified several proteins that shed further light on the distinctive pathogenesis of HA and could serve for biomarker research. However, these results need to be validated on a larger patient group.<\/jats:p><\/jats:sec>","DOI":"10.1002\/hsr2.70046","type":"journal-article","created":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T15:41:14Z","timestamp":1727278874000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Proteomic exploration of potential blood biomarkers in haemophilic arthropathy"],"prefix":"10.1002","volume":"7","author":[{"given":"Nata\u0161a","family":"Kalebota","sequence":"first","affiliation":[{"name":"Department of Rheumatology and Rehabilitation University Hospital Centre Zagreb Zagreb Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ru\u0111er","family":"Novak","sequence":"additional","affiliation":[{"name":"Centre for Translational and Clinical Research, Department of Proteomics University of Zagreb, School of Medicine Zagreb Croatia"},{"name":"BIMIS \u2013 Biomedical Research Centre \u0160alata University of Zagreb School of Medicine Zagreb Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stela","family":"Hrka\u010d","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology Allergology and Rheumatology, University Hospital Dubrava Zagreb Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Porin","family":"Peri\u0107","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Rehabilitation University Hospital Centre Zagreb Zagreb Croatia"},{"name":"University of Zagreb, School of Medicine Zagreb Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grgur","family":"Salai","sequence":"additional","affiliation":[{"name":"Department of Pulmonology University Hospital Dubrava Zagreb Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marko","family":"Mo\u010dibob","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Zagreb, Faculty of Science Zagreb Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marija","family":"Pranji\u0107","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Zagreb, Faculty of Science Zagreb Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zbyn\u011bk","family":"Zdr\u00e1hal","sequence":"additional","affiliation":[{"name":"Central European Institute of Technology Masaryk University, Kamenice 5 Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e1clav","family":"Pustka","sequence":"additional","affiliation":[{"name":"Central European Institute of Technology Masaryk University, Kamenice 5 Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadica Lakta\u0161i\u0107","family":"\u017derjavi\u0107","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Rehabilitation University Hospital Centre Zagreb Zagreb Croatia"},{"name":"University of Zagreb, School of Medicine Zagreb Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milan","family":"Milo\u0161evi\u0107","sequence":"additional","affiliation":[{"name":"Andrija \u0160tampar School of Public Health University of Zagreb, School of Medicine Zagreb Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marijo","family":"Vodanovi\u0107","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Internal Medicine University Hospital Centre Zagreb Zagreb Croatia"},{"name":"University of Applied Health Sciences Zagreb Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silva Zupan\u010di\u0107","family":"\u0160alek","sequence":"additional","affiliation":[{"name":"Department of Hematology Clinical Hospital \u201cSv. 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