{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T19:16:59Z","timestamp":1783970219484,"version":"3.55.0"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T00:00:00Z","timestamp":1739232000000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004320","name":"Shiraz University of Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004320","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Health Science Reports"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background and Aims<\/jats:title><jats:p>Viral infections are significant contributors to infertility. This study aimed to determine the prevalence of Herpes Simplex Virus 1 and 2 (HSV\u20101\/HSV\u20102) and cytomegalovirus (CMV) infections in men with infertility, compared to a control group of fertile men.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A cross\u2010sectional study was conducted with seminal fluid samples from 100 fertile and 100 infertile men, aged 20\u201340 years, referred to Yazd Infertility Center. DNA extraction was done using the AmpliSens kit, and the presence of CMV and HSV\u20101\/HSV\u20102 was determined via real\u2010time PCR. Sperm parameters (count, quick &amp; slow, motility, morphology, and volume) were also assessed and compared to the control group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CMV infection was found in 16% of fertile and 10% of infertile men. HSV\u20101\/HSV\u20102 prevalence was significantly higher in infertile men (32%) compared to fertile men (8%). Abnormal sperm count was significantly associated with HSV status (<jats:italic>p<\/jats:italic>\u2009=\u20090.001), as was abnormal morphology (<jats:italic>p<\/jats:italic>\u2009=\u20090.021), while abnormal quick &amp; slow, motility and volume showed no significant association. Data analysis indicated no correlation between sperm parameters\u2014including sperm quick &amp; slow, motility, count, morphology, and semen volume\u2014and CMV infection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>HSV infection is linked to sperm\u2010specific parameters, particularly count and morphology. These findings enhance our understanding of viral\u2010induced male infertility and underscore the need for targeted approaches to manage HSV\u20101\/HSV\u20102\u2010related infertility.<\/jats:p><\/jats:sec>","DOI":"10.1002\/hsr2.70221","type":"journal-article","created":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T13:48:44Z","timestamp":1739281724000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Prevalence of HSV\u20101\/HSV\u20102 and CMV Infections in Infertile Men and Their Impact on Sperm Parameters: A Cross\u2010Sectional Study in Iran"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7827-1260","authenticated-orcid":false,"given":"Seyed Mansour","family":"Salar","sequence":"first","affiliation":[{"name":"Department of Bacteriology &amp; Virology, School of Medicine Shiraz University of Medical Sciences Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5066-5279","authenticated-orcid":false,"given":"Fahime","family":"Edalat","sequence":"additional","affiliation":[{"name":"Autophagy Research Center Shiraz University of Medical Science Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0415-0239","authenticated-orcid":false,"given":"Arash","family":"Letafati","sequence":"additional","affiliation":[{"name":"Department of Virology, Faculty of Public Health Tehran University of Medical Sciences Tehran Iran"},{"name":"Research Center for Clinical Virology Tehran University of Medical Science Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6471-0157","authenticated-orcid":false,"given":"Seyed Mehdi","family":"Kalantar","sequence":"additional","affiliation":[{"name":"Reproductive &amp; 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