{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T13:12:07Z","timestamp":1790255527821,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T00:00:00Z","timestamp":1749600000000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Health Science Reports"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Aims<\/jats:title>\n                    <jats:p>\n                      Multidrug\u2010resistant (MDR) pathogens, particularly members of the ESKAPE group and\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      (collectively referred to as ESKAPEEc), are major contributors to bloodstream infections (BSIs) and pose significant treatment challenges. This study aimed to characterize the antimicrobial resistance (AMR) profiles of ESKAPEEc isolates from BSIs in public hospitals in the uMgungundlovu District, South Africa, and to assess their resistance to World Health Organization (WHO) Access, Watch, and Reserve (AWaRe) antibiotics.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Between November 2017 and December 2018, blood samples (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u2009195) were collected from adult and paediatric patients with suspected BSIs. Isolates were identified using the VITEK 2 system and confirmed by polymerase chain reaction (PCR). Antimicrobial susceptibility testing was performed using the Kirby\u2013Bauer disk diffusion method and interpreted according to EUCAST\/CLSI guidelines. The multiple antibiotic resistance index (MARI) was calculated. One\u2010way analysis of variance (ANOVA) was used to assess associations between MARI and clinical variables, including ward type and facility level.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Out of 195 presumptive isolates, 159 were confirmed as ESKAPEEc. The most frequently identified pathogens were\n                      <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                      (28.9%) and\n                      <jats:italic>Staphylococcus aureus<\/jats:italic>\n                      (28.3%). High resistance rates were observed across WHO Access and Watch antibiotics, including ampicillin (76% in\n                      <jats:italic>E. coli<\/jats:italic>\n                      ), gentamicin (67.4% in\n                      <jats:italic>K. pneumoniae<\/jats:italic>\n                      ), and ciprofloxacin (\u2265\u200960% in most species). Carbapenem resistance in\n                      <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                      reached 90%. Overall, 94.9% of isolates were MDR, and 93.1% had MARI\u2009\u2265\u20090.2. Significant differences in MARI values were observed across ward groups and facility levels, with the highest values recorded in intensive care units (mean\u2009=\u20090.67, 95% CI: 0.62\u20130.72) and tertiary hospitals (mean\u2009=\u20090.64, 95% CI: 0.60\u20130.68), compared to regional hospitals (mean\u2009=\u20090.52, 95% CI: 0.47\u20130.57).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The findings reveal a high burden of MDR ESKAPEEc in BSIs and widespread resistance to WHO Watch antibiotics. Targeted antimicrobial stewardship and the implementation of microbiology\u2010guided therapy are urgently needed to optimize patient outcomes and curb the spread of resistance.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/hsr2.70897","type":"journal-article","created":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T23:58:02Z","timestamp":1749686282000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Multidrug\u2010Resistant ESKAPEEc Pathogens From Bloodstream Infections in South Africa: A Cross\u2010Sectional Study Assessing Resistance to WHO AWaRe Antibiotics"],"prefix":"10.1002","volume":"8","author":[{"given":"Bakoena A.","family":"Hetsa","sequence":"first","affiliation":[{"name":"Antimicrobial Research Unit, College of Health Sciences University of KwaZulu\u2010Natal Durban South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Asante","sequence":"additional","affiliation":[{"name":"Antimicrobial Research Unit, College of Health Sciences University of KwaZulu\u2010Natal Durban South Africa"},{"name":"School of Pharmacy and Pharmaceutical Sciences University of Cape Coast  Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3551-3458","authenticated-orcid":false,"given":"Daniel G.","family":"Amoako","sequence":"additional","affiliation":[{"name":"Antimicrobial Research Unit, College of Health Sciences University of KwaZulu\u2010Natal Durban South Africa"},{"name":"Department of Pathobiology University of Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akebe L. 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