{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T23:47:52Z","timestamp":1778802472541,"version":"3.51.4"},"reference-count":37,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:00:00Z","timestamp":1761004800000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Health Science Reports"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Aims<\/jats:title>\n                    <jats:p>Forkhead box O (FoxO) transcription factors exert crucial roles in immune responses. Besides, the Runt\u2010related transcription factor (Runx) family plays a pivotal role in the development and homeostasis of cartilage and bone. This study assessed the plasma concentrations of the pro\u2010inflammatory interleukin\u20106 (IL\u20106) cytokine and the anti\u2010inflammatory cytokine interleukin\u201010 (IL\u201010), as well as the gene expression levels of FoxO1, FoxO3, Runx1, and Runx3, in the peripheral blood sample of the early rheumatoid arthritis (RA) patients treated with conventional disease\u2010modifying antirheumatic drugs (DMARDs) for 6\u2009months relative to normal subjects.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This study examined 30 early DMARDs\u2010na\u00efve RA patients (before and after treatment) and 30 age\u2010 and gender\u2010matched normal individuals. The plasma levels of IL\u20106 and IL\u201010 were measured by enzyme\u2010linked immunosorbent assay (ELISA), and the mRNA expression amounts of FoxO1, FoxO3, Runx1, and Runx3 were determined using real\u2010time PCR.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The plasma IL\u20106 concentrations increased significantly in pre\u2010 and posttreatment RA patients compared with controls (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). Moreover, the gene expression of FoxO1 and FoxO3 was enhanced considerably in RA patients (both before and after treatment), compared to controls (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001 for FoxO1 and\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.02 and\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.01 for FoxO3, respectively). Runx1 gene expression increased dramatically in pre\u2010 and posttreatment RA patients compared with the controls (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). Runx3 gene expression was meaningfully enhanced in pretreatment RA patients than in normal controls (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). Also, DMARDs treatment strikingly reduced Runx3 gene expression levels compared to pretreatment levels (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>DMARDs treatment dramatically diminishes Runx3 gene expression, thereby lowering disease activity and inflammatory markers in the early RA patients.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/hsr2.71388","type":"journal-article","created":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T05:14:16Z","timestamp":1761110056000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Efficacy of DMARDs Therapy on the Disease Activity and Gene Expression Levels of FoxO1, FoxO3, Runx1, and Runx3 in Early Rheumatoid Arthritis Patients: A Pre\u2013Post Interventional Study With Healthy Controls"],"prefix":"10.1002","volume":"8","author":[{"given":"Seyed Askar","family":"Roghani","sequence":"first","affiliation":[{"name":"Department of Anatomical Sciences, Faculty of Medical Sciences Tarbiat Modares University Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramin","family":"Lotfi","sequence":"additional","affiliation":[{"name":"Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine Tehran Iran"},{"name":"Clinical Research Development Center, Tohid Hospital Kurdistan University of Medical Sciences Sanandaj Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shirin","family":"Assar","sequence":"additional","affiliation":[{"name":"Clinical Research Development Center, Imam Reza Hospital Kermanshah University of Medical Sciences Kermanshah Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahdi","family":"Taghadosi","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Center, Health Technology Institute Kermanshah University of Medical Sciences Kermanshah Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seyedeh Zahra","family":"Shahrokhvand","sequence":"additional","affiliation":[{"name":"Clinical Research Development Center, Imam Reza Hospital Kermanshah University of Medical Sciences Kermanshah Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ghazal Hosseini","family":"Torshizi","sequence":"additional","affiliation":[{"name":"Department of Biology, Mashhad Branch Islamic Azad University Mashhad Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehran","family":"Pournazari","sequence":"additional","affiliation":[{"name":"Clinical Research Development Center, Imam Reza Hospital Kermanshah University of Medical Sciences 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