{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T12:47:02Z","timestamp":1776170822969,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T00:00:00Z","timestamp":1764115200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T00:00:00Z","timestamp":1764115200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Health Science Reports"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Aims<\/jats:title>\n                    <jats:p>Respiratory syncytial virus (RSV) is a leading cause of acute respiratory infection (ARI) in children, particularly in low\u2010 and middle\u2010income countries. Rapid diagnosis is important for management and infection control. We aimed to evaluate diagnostic accuracy of Sofia 2 RSV fluorescent immunoassay (FIA) compared to reverse\u2010transcription quantitative PCR (RT\u2010qPCR) in children under 2 years hospitalized with ARI in Amman, Jordan.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We conducted a prospective viral surveillance study from January 26, 2020 to March 17, 2020, at Al\u2010Bashir Hospital. Sofia 2 RSV FIA was performed on nasal swabs and compared to RT\u2010qPCR. We calculated sensitivity, specificity, and Cohen's\n                      <jats:italic>\u03ba<\/jats:italic>\n                      with 95% confidence intervals and assessed factors associated with RSV detection by FIA using logistic regression.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Of 458 children enrolled, 356 (77.7%) had RSV detected by RT\u2010qPCR. Sofia 2 RSV FIA demonstrated 76.1% sensitivity (95% CI: 71.3, 80.5%) and 94.1% specificity (95% CI: 87.6, 97.8%) relative to RT\u2010qPCR. Lower RT\u2010qPCR cycle threshold value (inversely related to viral load) was significantly associated with Sofia 2 RSV FIA positivity (aOR\u2009=\u20090.75; 95% CI: 0.70, 0.81;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Sofia 2 RSV FIA demonstrates potential as a rapid diagnostic test in resource\u2010limited settings. Further research on its clinical outcomes and cost\u2010effectiveness could enhance RSV management in children.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/hsr2.71576","type":"journal-article","created":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T06:13:51Z","timestamp":1764224031000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparison of Sofia 2 Respiratory Syncytial Virus Fluorescent Immunoassay to Real\u2010Time Polymerase Chain Reaction Assay in Children Under Two Years Old Hospitalized With Acute Respiratory Illness in Amman, Jordan: A Cross\u2010Sectional Study"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3703-5536","authenticated-orcid":false,"given":"Olla","family":"Hamdan","sequence":"first","affiliation":[{"name":"Department of Pediatrics Vanderbilt University Medical Center Nashville Tennessee 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