{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T18:25:38Z","timestamp":1787250338781,"version":"build-2736575974"},"reference-count":45,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T00:00:00Z","timestamp":1772582400000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Health Science Reports"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Toxoplasma gondii<\/jats:italic>\n                      (\n                      <jats:italic>T. gondii<\/jats:italic>\n                      ) infection poses significant risks during pregnancy, yet data on seroprevalence and genotype diversity in Ethiopia are scarce.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      This study aimed to determine the seroprevalence, associated risk factors, and genotype distribution of\n                      <jats:italic>T. gondii<\/jats:italic>\n                      among pregnant women in Northwest Ethiopia.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A cross\u2010sectional study was conducted among 554 pregnant women attending antenatal care in public hospitals between January 2022 and April 2024. Systematic random sampling was used. ELISA detected\n                      <jats:italic>T. gondii<\/jats:italic>\n                      \u2010specific IgG and IgM antibodies, and PCR\u2010RFLP targeting B1 and SAG2 loci were used for genotyping.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Overall seroprevalence was 54.3%, with 6.1% testing IgG\u2010positive only, 9.4% IgM\u2010positive only, and 38.8% positive for both. Cat ownership (AOR\u2009=\u20092.2; 95% CI: 1.4\u20133.5) and dog ownership (AOR\u2009=\u20094.9; 95% CI: 2.9\u20138.1) were significantly associated with infection. Among 28 IgM\u2010positive samples, Type II strains predominated (50%), followed by Types I and III (25% each).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These findings support targeted screening and pet\u2010handling education to reduce the risk of congenital toxoplasmosis. Further molecular surveillance is recommended to inform public health strategies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/hsr2.71918","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T11:50:07Z","timestamp":1772970607000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Seroprevalence and Genotype Distribution of\n                    <i>Toxoplasma gondii<\/i>\n                    Among Pregnant Women in Northwest Ethiopia: A Cross\u2010Sectional Study"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-7704-6341","authenticated-orcid":false,"given":"Eden","family":"Woldegerima","sequence":"first","affiliation":[{"name":"Department of Medical Biotechnology, Institutes of Biotechnology University of Gondar Gondar 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