{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T19:18:35Z","timestamp":1787426315258,"version":"build-2736575974"},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T00:00:00Z","timestamp":1773100800000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012151","name":"Sanming Project of Medicine in Shenzhen","doi-asserted-by":"publisher","award":["SZSM202211033"],"award-info":[{"award-number":["SZSM202211033"]}],"id":[{"id":"10.13039\/501100012151","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Health Science Reports"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Aims<\/jats:title>\n                    <jats:p>Children's coagulation system undergoes dynamic changes during development, necessitating age\u2010specific reference intervals (RIs). This study aimed to establish RIs for 16 coagulation parameters in Chinese children aged 0\u201318 years using the Sysmex CN\u20106000 analyzer.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We conducted a cross\u2010sectional study in children aged 0 day to 18 years who met the inclusion and exclusion criteria. Participants were stratified into six age groups (0\u201330 days, 1\u20136 months, 6\u201312 months, 1\u20133 years, 3\u201312 years, and 12\u201318 years) and blood samples were collected. Routine parameters (APTT, PT, PT\u2010INR, TT, Fibrinogen [Fib]), thrombotic markers (AT\u2010III, D\u2010Dimer [D\u2010Di], Fibrin Degradation Products [FDP]), and coagulation factors (II, V, VII, VIII, IX, X, XI, XII) were analyzed using the Sysmex CN\u20106000 analyzer. RIs and their 90% confidence intervals (CIs) were calculated using the non\u2010parametric method (for\n                      <jats:italic>n<\/jats:italic>\n                      \u2009\u2265\u2009120) or the robust method (for\n                      <jats:italic>n<\/jats:italic>\n                      \u2009&lt;\u2009120).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Neonates exhibited distinct coagulation profiles, including prolonged APTT (22.7\u2009s\u201346.5\u2009s) and TT (16.2\u2009s\u201320.8\u2009s), reduced levels of anticoagulant and coagulation factors (e.g., AT\u2010III: 23.1%\u201391.3%, factor II: 26.5%\u201377.5%), and elevated fibrinolytic markers (D\u2010Di \u2264\u20091.41\u2009\u03bcg\/mL; FDP\u2009\u2264\u20094.44\u2009\u03bcg\/mL). Age\u2010dependent trends were evident: APTT shortened until adolescence (24.7\u2009s\u201332.9\u2009s), while factor IX increased progressively (16.3% \u2013 43.2% in neonates vs. 43.6%\u2013108.6% in adolescents). Novel RIs for neonatal D\u2010Di and FDP were established, addressing a critical gap in developmental hemostasis. Sex\u2010specific differences in TT were observed but deemed clinically insignificant.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Age\u2010specific RIs are essential for children. This study provides the first comprehensive RIs for Sysmex CN\u20106000 in Chinese children, aiding in the diagnosis and management of coagulation disorders in pediatrics.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/hsr2.72023","type":"journal-article","created":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T04:32:02Z","timestamp":1773203522000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Establishment of Age\u2010Specific Reference Intervals for 16 Coagulation Parameters in Chinese Children Using Sysmex CN\u20106000 Analyzer: A Cross\u2010Sectional Study"],"prefix":"10.1002","volume":"9","author":[{"given":"Liping","family":"Luo","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine Shenzhen Children's Hospital Guangdong Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuyue","family":"Duan","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Shenzhen Children's Hospital Guangdong Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Shenzhen Children's Hospital Guangdong Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5906-3303","authenticated-orcid":false,"given":"Dong","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Shenzhen Children's Hospital Guangdong Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaxian","family":"Kuang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Shenzhen Children's Hospital Guangdong Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hematology &amp; Oncology Shenzhen Children's Hospital Guangdong Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo R.","family":"Brand\u00e3o","sequence":"additional","affiliation":[{"name":"Division of Hematology\u2010Oncology, Department of Paediatrics University of Toronto, The Hospital for Sick Children Ontario Toronto Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4210-3510","authenticated-orcid":false,"given":"Xiaoying","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Shenzhen Children's Hospital Guangdong Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,10]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.siny.2011.07.007"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/00043426-199021000-00019"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12519-021-00492-3"},{"issue":"1","key":"e_1_2_12_5_1","first-page":"9","article-title":"Age Dependency for Coagulation Parameters in Paediatric Populations. Results of a Multicentre Study Aimed at Defining the Age\u2010Specific Reference Ranges","volume":"116","author":"Toulon P.","year":"2016","journal-title":"Thrombosis and Haemostasis"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2018.04.003"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2005.050211"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.13098"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2019.11.005"},{"issue":"1","key":"e_1_2_12_10_1","first-page":"39","article-title":"The Investigation of Reference Intervals Application Status of the Analytes in Coagulation Test","volume":"36","author":"Wang W.","year":"2015","journal-title":"Zhonghua Xue Ye Xue Za Zhi = Zhonghua Xueyexue Zazhi"},{"key":"e_1_2_12_11_1","unstructured":"CLSI Collection Transport and Processing of Blood Specimens for Testing Plasma\u2010Based Coagulation Assays 6th ed. 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