{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T18:25:59Z","timestamp":1787250359814,"version":"build-2736575974"},"reference-count":64,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T00:00:00Z","timestamp":1773705600000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Health Science Reports"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Aims<\/jats:title>\n                    <jats:p>Influenza remains a globally prevalent respiratory infection that is challenging to diagnose accurately. This systematic review focuses on aptamer\u2010based tools for influenza virus detection, aiming to overcome the limitations of traditional diagnostic methods.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Following PRISMA guidelines, we identified relevant studies from official databases (PubMed, Web of Science, Scopus, Embase, Google Scholar) and grey literature sources. The search strategy utilized keywords related to aptamers, systematic evolution of ligands by exponential enrichment (SELEX) techniques, and influenza. The qualitative synthesis included 22 articles out of 1432 studies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Our analysis revealed diverse aptamer\u2010based techniques for influenza detection, including fluorescence resonance energy transfer (FRET)\u2010based aptasensors, electrochemical sensors, and surface\u2010enhanced raman scattering (SERS)\u2010based methods. SELEX was the predominant method for aptamer development, ensuring high specificity and adaptability to various influenza strains. Notably, FRET\u2010based methods demonstrated detection limits as low as 0.43\u2009ng\/mL, while electrochemical approaches exhibited high sensitivity with low detection limits in the picomolar range. The findings highlight the advantages of aptamers, including cost\u2010effectiveness, stability, and rapid synthesis, though challenges related to reproducibility and technological complexity remain.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Aptamer\u2010based tools offer promising alternatives to traditional diagnostics, especially for rapid and field\u2010deployable influenza detection. Their adaptability and potential for multiplex detection underscore their role in advancing public health diagnostics. Further research should focus on optimizing sensitivity and simplifying technological requirements for broader clinical applications.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/hsr2.72118","type":"journal-article","created":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T04:42:28Z","timestamp":1773808948000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Aptamer\u2010Based Approaches for Influenza Virus Detection: A Systematic Review"],"prefix":"10.1002","volume":"9","author":[{"given":"Javad","family":"Charostad","sequence":"first","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine Shahid Sadoughi University of Medical Sciences  Yazd Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zohreh\u2010al\u2010sadat","family":"Ghoreshi","sequence":"additional","affiliation":[{"name":"Student Research Committee Jiroft University of Medical Sciences  Jiroft Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7282-3538","authenticated-orcid":false,"given":"Nasir","family":"Arefinia","sequence":"additional","affiliation":[{"name":"Student Research Committee Jiroft University of Medical Sciences  Jiroft Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4368-779X","authenticated-orcid":false,"given":"Faranak","family":"Salajegheh","sequence":"additional","affiliation":[{"name":"Social Determinants of Health Research Center, Institute for Futures Studies in Health Kerman University of Medical Sciences  Kerman Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-5488-6969","authenticated-orcid":false,"given":"Niloofar","family":"Farsiu","sequence":"additional","affiliation":[{"name":"Gastroenterology and Hepatology Research Center, Institute of Basic and Clinical Physiology Sciences Kerman University of Medical Sciences  Kerman Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8390-4344","authenticated-orcid":false,"given":"Mohammad","family":"Rezaei Zadeh Rukerd","sequence":"additional","affiliation":[{"name":"HIV\/STI Surveillance Research Center, and WHO Collaborating Center for HIV Surveillance, Institute for Futures Studies in Health Kerman University of Medical Sciences  Kerman Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7605-2593","authenticated-orcid":false,"given":"Mohsen","family":"Nakhaie","sequence":"additional","affiliation":[{"name":"Research Center of Tropical and Infectious Diseases Kerman University of Medical Sciences  Kerman Iran"},{"name":"Clinical Research Development Unit, Afzalipour Hospital Kerman University of Medical Sciences  Kerman Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,17]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-017-0007-9"},{"key":"e_1_2_11_3_1","doi-asserted-by":"crossref","unstructured":"M.Yadav R.Singh andY.Malik2019. 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