{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T10:19:35Z","timestamp":1788344375239,"version":"build-2803163510"},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T00:00:00Z","timestamp":1776988800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T00:00:00Z","timestamp":1776988800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Health Science Reports"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Aims<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                      is a non\u2010fermentative, opportunistic Gram\u2010negative bacterium that causes 10%\u201315% of nosocomial and burn wound infections worldwide. This bacterium is resistant to various antibiotics through multiple mechanisms, including acquisition of resistance genes. The aim of this study was to perform a phenotypic and genotypic analysis of\n                      <jats:italic>blaPER\u20101<\/jats:italic>\n                      ,\n                      <jats:italic>blaVEB<\/jats:italic>\n                      , and\n                      <jats:italic>blaSHV\u20101<\/jats:italic>\n                      genes in clinical isolates of\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      One hundred and ten\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      isolates were collected from clinical specimens. The pattern of antimicrobial resistance was determined using the disk diffusion method. Phenotypic analysis of ESBL production was performed using the combined disk method. Additionally, molecular identification of\n                      <jats:italic>blaPER\u20101<\/jats:italic>\n                      ,\n                      <jats:italic>blaVEB<\/jats:italic>\n                      , and\n                      <jats:italic>blaSHV\u20101<\/jats:italic>\n                      \u03b2\u2010lactamase genes was carried out by polymerase chain reaction (PCR).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The highest resistance and sensitivity were observed against ceftazidime (86.36%) and colistin (78.18%), respectively. Among the 110 isolates, 72 were phenotypically ESBL\u2010producing. The\n                      <jats:italic>blaPER\u20101<\/jats:italic>\n                      gene was detected in 33 isolates (45.83%),\n                      <jats:italic>blaVEB<\/jats:italic>\n                      in 18 isolates (25.00%), and\n                      <jats:italic>blaSHV\u20101<\/jats:italic>\n                      in 27 isolates (37.50%).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Overall, phenotypic analysis indicated that 65.45% of isolates were ESBL producers, and the presence of\n                      <jats:italic>blaPER\u20101<\/jats:italic>\n                      ,\n                      <jats:italic>blaVEB<\/jats:italic>\n                      , and\n                      <jats:italic>blaSHV\u20101<\/jats:italic>\n                      genes was associated with increased resistance to ceftazidime, amikacin, ciprofloxacin, cefepime, and gentamicin. However, further studies are needed on ESBL\u2010producing isolates, as well as the identification of effective antibiotics for the treatment of\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      infections.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/hsr2.72329","type":"journal-article","created":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T03:55:25Z","timestamp":1777089325000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phenotypic and Genotypic Study of Antibiotic Resistance\n                    <i>blaPER\u20101<\/i>\n                    ,\n                    <i>blaVEB<\/i>\n                    , and\n                    <i>blaSHV\u20101<\/i>\n                    Genes in Clinical Isolates of\n                    <i>Pseudomonas aeruginosa<\/i>"],"prefix":"10.1002","volume":"9","author":[{"given":"Milad Gholampour","family":"Matin","sequence":"first","affiliation":[{"name":"Department of Microbiology, Faculty of Basic Sciences, Zanjan Branch Islamic Azad University Zanjan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1998-759X","authenticated-orcid":false,"given":"Reza","family":"Shapouri","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Basic Sciences, Zanjan Branch Islamic Azad University Zanjan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammadreza","family":"Nahaei","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Laboratory Sciences, Faculty of Medicine, Tabriz Medical Sciences Islamic Azad University Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mojtaba","family":"Mohammadi Roknabadi","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Basic Sciences, Zanjan Branch Islamic Azad University Zanjan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rasoul","family":"Shokri","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Basic Sciences, Zanjan Branch Islamic Azad University Zanjan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,24]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.005011"},{"issue":"3","key":"e_1_2_14_3_1","first-page":"1","article-title":"Synergistic or Antagonistic Effects of Probiotics and Antibiotics\u2010Alone or in Combination\u2010On Antimicrobial\u2010Resistant Pseudomonas aeruginosa Isolated From Burn Wounds","volume":"13","author":"Moghadam S. 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