{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T10:02:36Z","timestamp":1789984956472,"version":"4.0.1"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T00:00:00Z","timestamp":1780963200000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Health Science Reports"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Aims<\/jats:title>\n                    <jats:p>\n                      Frequent blood donation can significantly increase the risk of iron deficiency, influenced by demographic, dietary, environmental, and genetic factors. This study aims to evaluate the association between iron deficiency and specific genetic variations (\n                      <jats:italic>HIF\u20101\u03b1<\/jats:italic>\n                      (rs11549465; P582S),\n                      <jats:italic>TMPRSS6<\/jats:italic>\n                      (rs855791; V736A), and\n                      <jats:italic>ABO<\/jats:italic>\n                      (rs651007)) to advance personalized health management strategies.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      This cross\u2010sectional study was conducted at the Iranian Blood Transfusion Organization (IBTO) between March and September 2023, targeting male donors registered as regular donors in the database (with a minimum of two donations in the past year and at least five donations over the past 3 years) aged 18\u201365. Strict inclusion criteria were applied to minimize potential confounding variables. To investigate genetic polymorphisms, we utilized techniques such as Sanger sequencing, allele\u2010specific PCR, and high\u2010resolution melt (HRM) analysis. Iron status was assessed by measuring key biochemical indicators\u2014serum ferritin, Iron, transferrin saturation (TSAT), and total iron\u2010binding capacity (TIBC)\u2014through ELISA and colorimetric assays. Statistical analyses were performed using R software (version 4.1.1). Methods included one\u2010way ANOVA, the Kruskal\u2010Wallis test, the Shapiro\u2010Wilk test, and independent samples\n                      <jats:italic>t<\/jats:italic>\n                      \u2010tests. All tests were two\u2010sided, with a significance level set at\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The rs11549465 polymorphism in\n                      <jats:italic>HIF\u20101\u03b1<\/jats:italic>\n                      was significantly associated with ferritin levels, with the CT genotype group exhibiting higher mean ferritin levels compared to the CC group (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.02; 61.13\u2009\u00b1\u200942.87\u2009ng\/mL vs. 44.60\u2009\u00b1\u200929.40\u2009ng\/mL). No significant associations were observed between rs855791 or rs651007 polymorphisms and biochemical indicators of iron status.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Genetic screening for the rs11549465 polymorphism could be integrated into donor management programs to personalize strategies for mitigating Iron deficiency risks. Additional research involving larger and more diverse groups is necessary to investigate interactions between genetic, dietary, and environmental factors.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/hsr2.72629","type":"journal-article","created":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T06:50:34Z","timestamp":1781074234000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Relationship of Genetic Variability (\n                    <i>HIF\u20101\u0391<\/i>\n                    (rs11549465; P582S),\n                    <i>TMPRSS6<\/i>\n                    (rs855791; V736A) and\n                    <i>ABO<\/i>\n                    (rs651007) and Iron Deficiency in Repeated Blood Donors in 2023: A Cross\u2010Sectional Study Towards Individualized Health Management"],"prefix":"10.1002","volume":"9","author":[{"given":"Mohammad Pouya","family":"Samiee","sequence":"first","affiliation":[{"name":"Hematology and Blood Banking, Blood Transfusion Research Center High Institute for Research and Education in Transfusion Medicine Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7715-5591","authenticated-orcid":false,"given":"Azita","family":"Chegini","sequence":"additional","affiliation":[{"name":"Anesthesiology. Assistant Professor of Blood Transfusion Research Center High Institute for Research and Education in Transfusion Medicine Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2755-1921","authenticated-orcid":false,"given":"Majid","family":"Shahabi","sequence":"additional","affiliation":[{"name":"Biotechnology, Blood Transfusion Research Center High Institute for Research and Education in Transfusion Medicine Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6986-1314","authenticated-orcid":false,"given":"Amir","family":"Teimourpour","sequence":"additional","affiliation":[{"name":"Biostatics, Biological Products and Blood Safety Research Center High Institute for Research and Education in Transfusion Medicine Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,9]]},"reference":[{"key":"e_1_2_13_2_1","first-page":"1","volume-title":"Modern Nutrition in Health and Disease: Eleventh Edition","author":"Ross A. 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