{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T10:02:42Z","timestamp":1789380162546,"version":"build-2803163510"},"reference-count":54,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T00:00:00Z","timestamp":1782604800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T00:00:00Z","timestamp":1782604800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Health Science Reports"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Alopecia areata (AA) is an autoimmune disorder characterized by hair loss due to immune\u2010mediated destruction of hair follicles. The polygenic nature of AA, influenced by genetic and environmental factors, complicates its pathogenesis. Advances in bioinformatics have facilitated understanding of AA's genetic basis, providing insights into key molecular pathways and regulatory mechanisms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>This study utilized the GEO database (dataset GSE111061) to analyze differentially expressed genes (DEGs) between healthy controls and AA patients. Functional enrichment and protein\u2010protein interaction (PPI) network analyzes identified hub genes, transcription factors (TFs), and miRNAs. Tools such as STRING, Cytoscape, and KEGG were employed to uncover key interactions and pathways.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>An aggregate of 5134 DEGs was discovered, with 2576 elevated genes constituting a PPI network. Key pathways included chemokine signaling and cytokine\u2010cytokine receptor interactions. Ten hub genes (e.g., CCL5, CCR7, and CXCL9) and top TFs (ZNF683, FOXP3, and TBX21) were identified. Additionally, 49 miRNAs were predicted to regulate AA\u2010related genes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This work focuses on critical molecular regulators involved in AA pathogenesis, such as TFs and miRNAs. These findings suggest possible treatment targets and shed light on the molecular mechanisms of AA.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/hsr2.72699","type":"journal-article","created":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T06:44:51Z","timestamp":1782715491000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Understanding the Molecular Landscape of Alopecia Areata: Key Genes, Regulatory Networks, and Therapeutic Targets"],"prefix":"10.1002","volume":"9","author":[{"given":"Ahmed Ibrahim","family":"AbdElneam","sequence":"first","affiliation":[{"name":"Department of Clinical Biochemistry, Department of Basic Medical Sciences, College of Medicine Shaqra University Dawadmi Saudi Arabia"},{"name":"Molecular Genetics and Enzymology Department, Human Genetics and Genome Research Institute National Research Centre Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6418-6647","authenticated-orcid":false,"given":"Mohammed Saleh","family":"Al\u2010Dhubaibi","sequence":"additional","affiliation":[{"name":"Departments of Dermatology, College of Medicine Shaqra University Dawadmi Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6582-907X","authenticated-orcid":false,"given":"Saleh Salem","family":"Bahaj","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Faculty of Medicine and Health Sciences Sana'a University Sana'a Yemen"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3074-1347","authenticated-orcid":false,"given":"Ghada Farouk","family":"Mohammed","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Venereology, and Sexology, Faculty of Medicine Suez Canal University Ismailia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lina Mohammed","family":"Atef","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Venereology, and Sexology, Faculty of Medicine Suez Canal University Ismailia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,28]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2017.11"},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12016-021-08883-0"},{"issue":"6","key":"e_1_2_14_4_1","first-page":"404","article-title":"Alopecia Areata. 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