{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T10:02:54Z","timestamp":1789380174084,"version":"build-2803163510"},"reference-count":38,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T00:00:00Z","timestamp":1782604800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T00:00:00Z","timestamp":1782604800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Health Science Reports"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Aims<\/jats:title>\n                    <jats:p>Multidrug resistance (MDR) is a major determinant of relapse\u2010associated mortality in pediatric acute lymphoblastic leukemia (pALL). Although miR\u2010326 downregulation has been associated with poor prognosis, its role in chemoresistance remains undefined. This study investigated the mechanistic contribution of miR\u2010326 to MDR in pALL.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      miR\u2010326 expression was quantified by RT\u2010qPCR in 58 fresh bone marrow samples and 14 paired diagnosis\u2013relapse slides from pALL patients. A multidrug\u2010resistant CD10\n                      <jats:sup>\u2212<\/jats:sup>\n                      CD34\n                      <jats:sup>\u2212<\/jats:sup>\n                      B\u2010cell acute lymphoblastic leukemia (B\u2010ALL) cell line was transfected with miR\u2010326 mimic, and drug sensitivity was assessed by MTT assay. Bioinformatic analyses identified ABCA2 and YY1 as potential targets of miR\u2010326, which were validated by RT\u2010qPCR, Western blotting, and dual\u2010luciferase reporter assays.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Low miR\u2010326 expression was associated with relapse and reduced 4\u2010year disease\u2010free survival. Restoration of miR\u2010326 expression enhanced chemosensitivity and downregulated ABCA2 and YY1 at both mRNA and protein levels. Reporter assays confirmed direct targeting of both genes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>miR\u2010326 attenuates MDR in pALL through direct suppression of ABCA2 and YY1, highlighting its potential as a therapeutic target for overcoming chemoresistance in pediatric B\u2010ALL.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/hsr2.72720","type":"journal-article","created":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T06:47:35Z","timestamp":1782715655000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Increased Expression of miR\u2010326 Mediates Chemosensitivity in Pediatric Acute Lymphoblastic Leukemia Through\n                    <i>ABCA2<\/i>\n                    and\n                    <i>YY1<\/i>\n                    Downregulation: An Observational and Experimental Study"],"prefix":"10.1002","volume":"9","author":[{"given":"Narges","family":"Aberuyi","sequence":"first","affiliation":[{"name":"University of Isfahan Isfahan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1376-255X","authenticated-orcid":false,"given":"Soheila","family":"Rahgozar","sequence":"additional","affiliation":[{"name":"University of Isfahan Isfahan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melika","family":"Katebi","sequence":"additional","affiliation":[{"name":"University of Isfahan Isfahan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,28]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers16111988"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2024.1394523"},{"key":"e_1_2_13_4_1","first-page":"15","volume-title":"Mechanisms of Multidrug Resistance in Cancer","author":"Aptamers Engineered Nanocarriers for Cancer Therapy","year":"2023"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/17425255.2021.1887139"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/15330338241273143"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/10428194.2018.1448083"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23031728"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/08880018.2020.1871139"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V114.22.4695.4695"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.26800"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-50628-w"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1089\/cbr.2020.3600"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2009.10.017"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.3109\/1061186X.2015.1015536"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-021-06161-y"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.2147\/CMAR.S214405"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.578229"},{"issue":"1","key":"e_1_2_13_19_1","first-page":"24","article-title":"Recovery of MicroRNA From Stored Bone Marrow Aspirate Slides","volume":"11","author":"Ghodousi E. 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