{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T07:54:23Z","timestamp":1790927663885,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T00:00:00Z","timestamp":1785110400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T00:00:00Z","timestamp":1785110400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Health Science Reports"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Dengue fever lacks reliable biomarkers to explain its complex pathophysiology. Substance P (SP) and its receptor, the Neurokinin\u20101 receptor (NK1R), known regulators of inflammation and vascular permeability in viral infections, may play a role in dengue but remain unexplored.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aim\/Objective<\/jats:title>\n                    <jats:p>To investigate the levels of SP and NK1R in serum samples of diagnosed dengue patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Participants were selected from a pool of diagnosed dengue fever patients using a convenience sampling technique (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200940), along with a control group of healthy individuals (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200912). Serum samples were collected, and SP and NK1R levels were measured using enzyme\u2010linked immunosorbent assay (ELISA). Complete blood count (CBC) parameters were also evaluated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The mean NK1R levels in the control and case groups were 2.681\u2009\u00b1\u20093.147 and 0.57\u2009\u00b1\u20090.131, respectively. NK1R levels were significantly lower in dengue patients compared with controls (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001). SP levels did not differ significantly between male and female dengue patients (Mann\u2013Whitney\n                      <jats:italic>U<\/jats:italic>\n                      \u2009=\u200985,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.899).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>NK1R may be associated with dengue pathogenesis through its potential involvement in vascular permeability, immune activation, and cytokine responses. These findings suggest a possible connection between NK1R and the pathophysiological mechanisms underlying dengue infection.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/hsr2.72820","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T08:11:06Z","timestamp":1785139866000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Substance P and NK\u20101R in Dengue: A First Serological Investigation"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8965-1587","authenticated-orcid":false,"given":"Riffat","family":"Mehboob","sequence":"first","affiliation":[{"name":"Rotogen Biotech Bethesda Maryland USA"},{"name":"Lahore Medical Research Center Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imran","family":"Shahid","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Faculty 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