{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T07:56:32Z","timestamp":1791186992265,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T00:00:00Z","timestamp":1787702400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T00:00:00Z","timestamp":1787702400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004320","name":"Shiraz University of Medical Sciences","doi-asserted-by":"publisher","award":["28149"],"award-info":[{"award-number":["28149"]}],"id":[{"id":"10.13039\/501100004320","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Health Science Reports"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Aims<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Toxoplasma gondii<\/jats:italic>\n                      is a widespread zoonotic protozoan that poses significant health risks, particularly to immunocompromised individuals. Although generally asymptomatic, toxoplasmosis can lead to severe neurological complications and behavioral disorders. This infection can be transmitted through various routes, including sharing needles and\/or syringes with someone who is infected. The current cross\u2010sectional study aimed to determine the prevalence of toxoplasmosis among drug users in Fars Province in the south of Iran.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Subjects of the study were 1400 drug users. Blood samples were collected from each individual from June 2023 to April 2024.\n                      <jats:italic>T. gondii<\/jats:italic>\n                      antigens were prepared from the parasite's tachyzoites and used in an ELISA system to detect anti\u2010\n                      <jats:italic>Toxoplasma<\/jats:italic>\n                      antibodies. The data were analyzed to explore the possible association between\n                      <jats:italic>Toxoplasma<\/jats:italic>\n                      seropositivity and participants' demographics.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The majority (52.7%) of the participants were aged 40\u201360\u2009years, with 83.4% living in urban areas. Males constituted 92.8% and females 7.2% of the subjects. Anti\u2010\n                      <jats:italic>Toxoplasma<\/jats:italic>\n                      antibodies were detected in the sera of 189 individuals, corresponding to a seroprevalence of 13.5% (95% CI, 9.5%\u201315.4%). Notably, 89.9% of those using injection methods for substance use were infected. Moreover, 94.2% of the infected individuals were male. Our findings revealed no association between\n                      <jats:italic>Toxoplasma<\/jats:italic>\n                      seropositivity and the demographic or method of drug use in patients with substance use disorder in Fars Province in the south of Iran.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Findings of the current study underscore the need for targeted public health interventions to address the risk of toxoplasmosis in this vulnerable population, particularly among those who use injection drugs. Further research is warranted to explore the underlying factors contributing to\n                      <jats:italic>Toxoplasma<\/jats:italic>\n                      infection in this population and to develop effective prevention strategies.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/hsr2.73057","type":"journal-article","created":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T05:31:41Z","timestamp":1787808701000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Serosurvey of Toxoplasmosis Among Drug Users in Fars Province, Southern Iran: A Cross\u2010Sectional Study"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-0620-1513","authenticated-orcid":false,"given":"Mohammad Reza","family":"Cheraghi","sequence":"first","affiliation":[{"name":"Student Research Committee Shiraz University of Medical Sciences Shiraz 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