{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,10]],"date-time":"2026-11-10T12:21:30Z","timestamp":1794313290449,"version":"4.9.0"},"reference-count":39,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Health Science Reports"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Aims<\/jats:title>\n                    <jats:p>Herpes simplex virus type 1 (HSV\u20101) is highly prevalent worldwide, making accurate serological testing essential for both clinical diagnosis and epidemiological surveillance. Automated chemiluminescent immunoassays (CLIAs) offer operational advantages over enzyme\u2010linked immunosorbent assays (ELISAs); however, their diagnostic performance relative to Western blot (WB) confirmation in high\u2010prevalence settings remains insufficiently characterized. The comparative diagnostic accuracy of CLIA\u2010 and ELISA\u2010based assays for HSV\u20101 IgG detection, when benchmarked against a WB reference standard in endemic populations, remains unclear. This study aimed to evaluate HSV\u20101 IgG seroprevalence and diagnostic performance of one CLIA and two ELISA platforms using Western blot as the reference method.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Four hundred archived serum samples from adult male craft and manual workers in Qatar were tested using the Mindray CL\u2010900i CLIA, HerpeSelect ELISA, NovaLisa ELISA, and Euroimmun Western blot. Seroprevalence, diagnostic accuracy, and interassay agreement were assessed using WB as the reference standard, with equivocal and indeterminate results excluded from analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      HSV\u20101 IgG seroprevalence estimates were comparable across assays: HerpeSelect 72.5% (290\/400), Mindray 70.3% (281\/400), NovaLisa 66.3% (265\/400), and Western blot 57.8% (231\/400), with no statistically significant differences (all\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&gt;\u20090.05). The Mindray CLIA demonstrated the highest diagnostic performance (sensitivity 95.7%, specificity 88.9%, accuracy 93.4%) and strong agreement with Western blot (\n                      <jats:italic>\u03ba<\/jats:italic>\n                      \u2009=\u20090.85). HerpeSelect showed substantial agreement (\n                      <jats:italic>\u03ba<\/jats:italic>\n                      \u2009=\u20090.81), while NovaLisa exhibited lower specificity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>CLIA\u2010 and ELISA\u2010based assays produced comparable HSV\u20101 seroprevalence estimates in this high\u2010prevalence population; however, diagnostic accuracy varied across platforms. The CLIA platform demonstrated the strongest agreement with Western blot, supporting its use in high\u2010throughput settings, while confirmatory testing remains important to minimize misclassification.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/hsr2.73366","type":"journal-article","created":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T06:33:54Z","timestamp":1791527634000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative Evaluation of CLIA and ELISA Serological Assays for HSV\u20101 IgG With Western Blot Confirmation in a Clinical Cohort: A Cross\u2010Sectional Study"],"prefix":"10.1002","volume":"9","author":[{"given":"Farah","family":"Issa","sequence":"first","affiliation":[{"name":"Department of Biomedical Science, College of Health Sciences Qatar University Doha Qatar"},{"name":"Biomedical Research Center Qatar University Doha Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3104-5781","authenticated-orcid":false,"given":"Farah","family":"Trad","sequence":"additional","affiliation":[{"name":"Biomedical Research Center Qatar University Doha Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3820-7157","authenticated-orcid":false,"given":"Nouran","family":"Zein","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, College of Health Sciences Qatar University Doha Qatar"},{"name":"Biomedical Research Center Qatar University Doha Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3308-8953","authenticated-orcid":false,"given":"Shaden","family":"Abunasser","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, College of Health Sciences Qatar University Doha Qatar"},{"name":"Biomedical Research Center Qatar University Doha Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parveen B.","family":"Nizamuddin","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, College of Health Sciences Qatar University Doha Qatar"},{"name":"Biomedical Research Center Qatar University Doha Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-9257-3617","authenticated-orcid":false,"given":"Israa","family":"Salameh","sequence":"additional","affiliation":[{"name":"Biomedical Research Center Qatar University Doha Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2512-6657","authenticated-orcid":false,"given":"Houssein","family":"Ayoub","sequence":"additional","affiliation":[{"name":"Mathematics Program, Department of Mathematics and Statistics, College of Arts and Sciences Qatar University Doha 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