{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T04:34:27Z","timestamp":1776832467276,"version":"3.51.2"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,12,11]],"date-time":"2022-12-11T00:00:00Z","timestamp":1670716800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Health Science Reports"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Aims<\/jats:title><jats:p>The transient receptor potential cationic channel ankyrin 1 (TRPA1), a channel protein permeable to most divalent cations, has been suggested to play a role in mechano\u2010afferent\/efferent signaling (including the release of neurotransmitters) in the human urinary tract (bladder, prostate, and urethra). To date, only a few studies have addressed the expression of this receptor in male and female reproductive tissues. The present study aimed to evaluate human seminal vesicles (SVs)\u00a0 for the expression and localization of TRPA1.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>SV tissue was obtained from 5 males who had undergone pelvic surgery due to malignancies of the prostate or urinary bladder. The expression of messenger ribonucleic acid (mRNA) specifically encoding for the TRPA1 protein was elucidated by means of reverse transcriptase polymerase chain reaction (RT\u2010PCR). Using immunohistochemical methods, the distribution of TRPA1 was examined in relation to the endothelial and neuronal nitric oxide synthases (eNOS, nNOS) and the neuropeptides calcitonin gene\u2010related peptide (CGRP) and vasoactive intestinal polypeptide (VIP).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>RT\u2010PCR revealed signals related to the expected molecular size of 656\u2009bp. Immunohistochemistry demonstrated that TRPA1 is located in nerves running through the smooth muscle portion of the SV. Here, the protein is in part co\u2010localized with nNOS and CGRP, whereas no co\u2010localization with VIP was registered. Dot\u2010like signals specific for TRPA1 were observed in the cytoplasm of epithelial cells lining the lumen of glandular spaces. The epithelial layer also presented staining for eNOS. The smooth musculature appeared free of immunosignals for TRPA1.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The results convincingly show the expression of TRPA1 in nerve endings as well as in epithelial cells of the SV. Based on its location in epithelial cells, TRPA1 might be involved in the mechanism of the NO\/cyclic guanosine monophosphate (GMP)\u2010mediated signaling and also the control of secretory function (mediated by cyclic GMP) in the human SV.<\/jats:p><\/jats:sec>","DOI":"10.1002\/hsr2.987","type":"journal-article","created":{"date-parts":[[2022,12,12]],"date-time":"2022-12-12T06:30:05Z","timestamp":1670826605000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Expression and distribution of the transient receptor potential cationic channel ankyrin 1 (TRPA1) in the human seminal vesicles"],"prefix":"10.1002","volume":"6","author":[{"given":"Harrina E.","family":"Rahardjo","sequence":"first","affiliation":[{"name":"Department of Urology, Faculty of Medicine, Cipto Mangunkusumo Hospital University of Indonesia Jakarta Indonesia"},{"name":"Department of Urology &amp; Urological Oncology, Hannover Medical School Division of Surgery Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7169-0385","authenticated-orcid":false,"given":"Stefan","family":"\u00dcckert","sequence":"additional","affiliation":[{"name":"Department of Urology &amp; Urological Oncology, Hannover Medical School Division of Surgery Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus A.","family":"Kuczyk","sequence":"additional","affiliation":[{"name":"Department of Urology, Faculty of Medicine, Cipto Mangunkusumo Hospital University of Indonesia Jakarta Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petter","family":"Hedlund","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology, Faculty of Medicine Link\u00f6ping University Link\u00f6ping Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,12,11]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2015.08.033"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03344077"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/edrv.22.3.0430"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2010.07.461"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2009.09.034"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1743-6109.2009.01423.x"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00240-003-0322-5"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-4295(02)01534-0"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cphy.c120001"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2010.09650.x"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2009.08.019"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/ijir.2017.15"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/ijir.2014.23"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbb.2013.12.004"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2008.07.063"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.258372"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22052783"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2007.10.024"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2010.202077"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins10080326"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41585-021-00428-6"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.12502"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2007.04.003"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.23338"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2009.12.093"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01268862"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.RA120.002174"}],"container-title":["Health Science Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/hsr2.987","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/hsr2.987","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/hsr2.987","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,24]],"date-time":"2023-08-24T07:29:46Z","timestamp":1692862186000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/hsr2.987"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,11]]},"references-count":27,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,1]]}},"alternative-id":["10.1002\/hsr2.987"],"URL":"https:\/\/doi.org\/10.1002\/hsr2.987","archive":["Portico"],"relation":{},"ISSN":["2398-8835","2398-8835"],"issn-type":[{"value":"2398-8835","type":"print"},{"value":"2398-8835","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,11]]},"assertion":[{"value":"2022-08-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-11-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e987"}}