{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T23:20:18Z","timestamp":1768519218424,"version":"3.49.0"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,9,18]],"date-time":"2022-09-18T00:00:00Z","timestamp":1663459200000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NSF 81601074"],"award-info":[{"award-number":["NSF 81601074"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ibrain"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study aimed to investigate the role of interleukin\u20106 (IL\u20106) in the pathogenesis of neonatal hypoxic\u2010ischemic encephalopathy (NHIE). Sprague\u2010Dawley (SD) rats were used for the establishment of hypoxic\u2010ischemic (HI) model. The Zea\u2010Longa scoring was used to evaluate the extent of the neurological deficits. Triphenyl tetrazolium chloride (TTC) staining was used to measure the volume of infarction in the brain following HI protocol. The expression of IL\u20106 in the cortex and\/or hippocampus at multiple time points after HI was examined by immunohistochemistry, western blotting and immunofluorescence. Moreover, small interfering RNAs (siRNA) were used to inhibit the expression of IL\u20106 in\u2010vitro and in\u2010vivo, and the concomitant expression of the Bcl\u20102 associated X protein (BAX) and caspase 3 was also measured. HI induced a significant brain damage, and these pathological changes were accompanied by IL\u20106 upregulation which was found localized in cortical neurons. The inhibition of IL\u20106 expression fostered neuronal and axonal growth, and a reduction in cellular apoptosis in cortical neuronal cultures, and cortex and hippocampus of neonatal rats. The expression of apoptotic markers such as BAX and caspase 3 was closely associated with IL\u20106. Downregulation of IL\u20106 could ameliorate HI\u2010induced deficiencies by mediating the expression of caspase 3 and BAX.<\/jats:p>","DOI":"10.1002\/ibra.12067","type":"journal-article","created":{"date-parts":[[2022,9,18]],"date-time":"2022-09-18T09:30:04Z","timestamp":1663493404000},"page":"413-428","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Knockdown of interleukin\u20106 plays a neuroprotective role against hypoxia\u2010ischemia in neonatal rats via inhibition of caspase 3 and Bcl\u20102\u2010associated X protein signaling pathway"],"prefix":"10.1002","volume":"8","author":[{"given":"Xiu","family":"Yang","sequence":"first","affiliation":[{"name":"Animal Model Research Center of Human Disease Kunming Medical University Kunming China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ke\u2010Han","family":"Liao","sequence":"additional","affiliation":[{"name":"School of Anesthesiology Southwest Medical University Luzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isaac B.","family":"Deng","sequence":"additional","affiliation":[{"name":"Health and Biomedical Innovation, Clinical and Health Sciences University of South Australia Adelaide South Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5965-1559","authenticated-orcid":false,"given":"Lan\u2010Chun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Animal Model Research Center of Human Disease Kunming Medical University Kunming 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