{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:54:32Z","timestamp":1753869272010,"version":"3.41.2"},"reference-count":32,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T00:00:00Z","timestamp":1698624000000},"content-version":"vor","delay-in-days":29,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82072864"],"award-info":[{"award-number":["82072864"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>This study was intended to investigate the effect of SAMD1 on antiphospholipid syndrome (APS)\u2010induced pregnancy complications in mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The mRNA and protein expression of SAMD1 in APS patients and healthy controls was detected by qRT\u2010PCR and western blot. Anti\u2010B<jats:sub>2<\/jats:sub>GPI and ACA levels were tested by ELISA, MMP\u20109, iNOS, ICAM\u20101 and MCP\u20101 mRNA and protein levels determined by qRT\u2010PCR and western blot, cellular senescence detected by \u03b2\u2010galactosidase staining, cell proliferation ability detected by CCK\u20108 assay, cell viability detected by trypan blue staining, cell mobility detected by Transwell, and cell angiogenesis ability detected by matrigel tube formation assay. An APS pregnant mouse model was constructed, and the embryo absorption rate was calculated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>SAMD1 expression was low in serum of APS patients, which was correlated with the history of thrombosis and the number of adverse pregnancies. Anti\u2010B<jats:sub>2<\/jats:sub>GPI and ACA levels were increased in APS. The expressions of MMP\u20109, iNOS, ICAM\u20101, and MCP\u20101 were also significantly upregulated in HUVECs treated with APS serum. APS promoted HUVEC senescence and inhibited cell proliferation, migration and angiogenesis. Overexpression of SAMD1 reversed the above results. Experiments on the APS pregnant mouse model confirmed that overexpression of SAMD1 reduced the rate of fetal loss.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>SAMD1 may reduce APS\u2010induced embryo loss by regulating cellular senescence, proliferation, migration, and angiogenesis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.1006","type":"journal-article","created":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T10:56:39Z","timestamp":1698663399000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["SAMD1 attenuates antiphospholipid syndrome\u2010induced pregnancy complications"],"prefix":"10.1002","volume":"11","author":[{"given":"Ran","family":"An","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology The Fourth Affiliated Hospital of Harbin Medical University Harbin Heilongjiang P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanqi","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The Fourth Affiliated Hospital of Harbin Medical University Harbin Heilongjiang P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The Fourth Affiliated Hospital of Harbin Medical University Harbin Heilongjiang P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1006-3825","authenticated-orcid":false,"given":"Peiling","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The Second Affiliated Hospital of Harbin Medical University Harbin Heilongjiang P.R. 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