{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T17:29:33Z","timestamp":1775064573288,"version":"3.50.1"},"reference-count":48,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,3,24]],"date-time":"2016-03-24T00:00:00Z","timestamp":1458777600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>To support dietary management of severe cow's milk allergic infants, a synbiotic mixture of non\u2010digestible oligosaccharides and <jats:italic>Bifidobacterium breve<\/jats:italic> M\u201016V (<jats:italic>B. breve<\/jats:italic>) was designed from source materials that are completely cow's milk\u2010free. It was investigated whether this specific synbiotic concept can reduce an established food allergic response in a research model for hen's egg allergy. Mice were orally sensitized once a week for 5 weeks to ovalbumin (OVA) using cholera toxin (CT) as an adjuvant. Non\u2010sensitized mice received CT in PBS only. Sensitized mice were fed a control diet or a diet enriched with short\u2010chain\u2010 (scFOS) and long\u2010chain fructo\u2010oligosaccharides (lcFOS), <jats:italic>B. breve<\/jats:italic> or scFOSlcFOS\u2009+\u2009<jats:italic>B. breve<\/jats:italic> for 3 weeks starting after the last sensitization. Non\u2010sensitized mice received the control diet. Anaphylactic shock symptoms, acute allergic skin responses and serum specific IgE, mMCP\u20101 and galectin\u20109 were measured upon OVA challenge. Activated Th2\u2010, Th1\u2010cells and regulatory T\u2010cells were quantified in spleen and mesenteric lymph nodes (MLN) and cytokine profiles were analyzed. Short chain fatty acids (SCFA) were measured in ceacal samples. The acute allergic skin response was reduced in mice fed the scFOSlcFOS\u2009+\u2009<jats:italic>B. breve<\/jats:italic> diet compared to mice fed any of the other diets. A reduction in mast cell degranulation (mMCP\u20101) and anaphylactic shock symptoms was also observed in these mice. Unstimulated splenocyte cultures produced increased levels of IL10 and IFNg in mice fed the scFOSlcFOS\u2009+\u2009<jats:italic>B. breve<\/jats:italic> diet. Correspondingly, increased percentages of activated Th1 cells were observed in the spleen. Allergen\u2010specific re\u2010stimulation of splenocytes showed a decrease in IL5 production. In summary; post\u2010sensitization administration of scFOSlcFOS\u2009+\u2009<jats:italic>B. breve<\/jats:italic> was effective in reducing allergic symptoms after allergen challenge. These effects coincided with changes in regulatory and effector T\u2010cell subsets and increases in the SCFA propionic acid. These results suggest immune modulatory benefits of dietary intervention with a unique combination of scFOSlcFOS\u2009+\u2009<jats:italic>B. breve<\/jats:italic> in established food allergy. Whether these effects translate to human applications is subject for ongoing clinical studies.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.101","type":"journal-article","created":{"date-parts":[[2016,3,24]],"date-time":"2016-03-24T12:48:54Z","timestamp":1458823734000},"page":"155-165","source":"Crossref","is-referenced-by-count":37,"title":["Post\u2010sensitization administration of non\u2010digestible oligosaccharides and <i>Bifidobacterium breve<\/i> M\u201016V reduces allergic symptoms in mice"],"prefix":"10.1002","volume":"4","author":[{"given":"Betty C. A. 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