{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T01:44:49Z","timestamp":1792719889214,"version":"4.5.0"},"reference-count":37,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T00:00:00Z","timestamp":1695772800000},"content-version":"vor","delay-in-days":26,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Fibroblast activation protein\u2010\u03b1 (FAP) and livin \u03b1 are considered as cancer\u2010associated fibroblasts (CAFs) and tumor\u2010specific targets, respectively, for immunogenic tumor vaccines. This study is designed to decipher the antitumor effect of double\u2010gene modified dendritic cells (DCs) on Lewis lung carcinoma (LLC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>By encoding mouse FAP cDNA and human livin \u03b1 (i.e., hlivin \u03b1) cDNA into recombinant adenoviral vector (rAd), rAd\u2010FAP, rAd\u2010hlivin \u03b1, and rAd\u2010FAP\/hlivin \u03b1 were constructed, which were then transduced into mouse DCs. LLC\u2010bearinig mice were immunized with the infected DCs (5\u2009\u00d7\u200910<jats:sup>5<\/jats:sup> cells\/mouse), followed by calculation of tumor volume and survival rate. The identification of CAFs from mouse LLC as well as the determination on expressions of FAP and livin \u03b1, was accomplished by western blot. Cytotoxic T lymphocyte assay was harnessed to assess the effect of the infected DCs on inducing splenic lymphocytes to lyse CAFs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>DCs were successfully transduced with rAd\u2010FAP\/hlivin \u03b1 in vitro. FAP was highly expressed in CAFs. CAFs were positive for \u03b1\u2010SMA and negative for CD45 and CD31. Livin \u03b1 level was upregulated in mouse LLC. Immunization with rAd\u2010FAP\/hlivin \u03b1\u2010transduced DCs suppressed LLC volume and improved the survival of tumor\u2010bearing mice. Immunization with rAd\u2010FAP\/hlivin \u03b1\u2010transduced DCs enhanced the cytotoxic effect of splenic lymphocytes on LLC tumor\u2010derived CAFs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Injection with rAd\u2010FAP\/hlivin \u03b1\u2010transduced DCs promotes immune\u2010enhanced tumor microenvironment by decreasing CAFs and suppresses tumor growth in LLC mouse models.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.1011","type":"journal-article","created":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T11:56:11Z","timestamp":1695815771000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Dendritic cells infected with recombinant adenoviral vector encoding mouse fibroblast activation protein\u2010\u03b1 and human livin \u03b1 exert an antitumor effect against Lewis lung carcinoma in mice"],"prefix":"10.1002","volume":"11","author":[{"given":"Zaiting","family":"Ye","sequence":"first","affiliation":[{"name":"Department of Radiology The Sixth Affiliated Hospital of Wenzhou Medical University  Lishui Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiongwei","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Respiratory The Sixth Affiliated Hospital of Wenzhou Medical University  Lishui Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhangyong","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Respiratory The Sixth Affiliated Hospital of Wenzhou Medical University  Lishui Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuanghu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Medicine Lishui People's Hospital  Lishui Zhejiang 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