{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T13:49:52Z","timestamp":1790776192873,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T00:00:00Z","timestamp":1697068800000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Chronic obstructive pulmonary disease (COPD) is an airway\u2010associated lung disorder, resulting in airway inflammation. This article aimed to explore the role of the kr\u00fcppel\u2010like factor 9 (KLF9)\/microRNA (miR)\u2010494\u20103p\/phosphatase and tensin homolog (PTEN) axis in airway inflammation and pave a theoretical foundation for the treatment of COPD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The COPD mouse model was established by exposure to cigarette smoke, followed by measurements of total cells, neutrophils, macrophages, and hematoxylin and eosin staining. The COPD cell model was established on human lung epithelial cells BEAS\u20102B using cigarette smoke extract. Cell viability was assessed by cell counting kit\u20108 assay. miR\u2010494\u20103p, KLF9, PTEN, and NLR family, pyrin domain containing 3 (NLRP3) levels in tissues and cells were measured by quantitative real\u2010time polymerase chain reaction or Western blot assay. Inflammatory factors (TNF\u2010\u03b1\/IL\u20106\/IL\u20108\/IFN\u2010\u03b3) were measured by enzyme\u2010linked immunosorbent assay. Interactions among KLF9, miR\u2010494\u20103p, and PTEN 3\u2032UTR were verified by chromatin immunoprecipitation and dual\u2010luciferase assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>KLF9 was upregulated in lung tissues of COPD mice. Inhibition of KLF9 alleviated airway inflammation, reduced intrapulmonary inflammatory cell infiltration, and repressed NLRP3 expression. KLF9 bound to the miR\u2010494\u20103p promoter and increased miR\u2010494\u20103p expression, and miR\u2010494\u20103p negatively regulated PTEN expression. miR\u2010494\u20103p overexpression or Nigericin treatment reversed KLF9 knockdown\u2010driven repression of NLRP3 inflammasome and inflammation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>KLF9 bound to the miR\u2010494\u20103p promoter and repressed PTEN expression, thereby facilitating NLRP3 inflammasome\u2010mediated inflammation.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.1043","type":"journal-article","created":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T07:38:27Z","timestamp":1697096307000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Mechanism of KLF9 in airway inflammation in chronic obstructive pulmonary"],"prefix":"10.1002","volume":"11","author":[{"given":"Peijie","family":"Gu","sequence":"first","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine Jiangyin Hospital of Traditional Chinese Medicine Jiangyin City China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine Jiangyin Hospital of Traditional Chinese Medicine Jiangyin City China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine Jiangyin Hospital of Traditional Chinese Medicine Jiangyin City China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine Jiangyin Hospital of Traditional Chinese Medicine 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