{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T18:44:08Z","timestamp":1773859448600,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,5,9]],"date-time":"2024-05-09T00:00:00Z","timestamp":1715212800000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Considering the antihepatitis effects of Tectorigenin (TEC), and the same adenosine mitogen\u2010activated protein kinase (MAPK) pathway in both hepatitis and inflammatory bowel disease (IBD) models, exploring the role of TEC in IBD is contributive to develop a new treatment strategy against IBD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The IBD mouse model was constructed by feeding with dextran sodium sulfate (DSS) and injection of TEC. Afterward, the mouse body weight, colon length, and disease activity index (DAI) were tested to assess the enteritis level. Mouse intestine lesions were detected by hematoxylin and eosin staining. Murine macrophages underwent lipopolysaccharide (LPS) induction to establish an inflammation model. Cell viability was determined by cell counting kit\u20108 assay. Enzyme\u2010linked immunosorbent assay was performed to measure interleukin 6\u00a0(IL\u20106) and tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1) levels. Cyclooxygenase\u20102\u00a0(COX\u20102) and inducible nitric oxide synthase\u00a0(iNOS) expressions were quantified via quantitative reverse transcription polymerase chain reaction. Levels of MAPK pathway\u2010related proteins (p\u2010P38, P38, p\u2010Jun N\u2010terminal kinase\u00a0(JNK), JNK, signal\u2010regulated kinase\u00a0(ERK), p\u2010ERK), COX\u20102 and iNOS were quantitated by Western blot.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>TEC improved the inflammatory response through ameliorating weight loss, shortening colon, and increasing DAI score in IBD mouse. Expressions of intestinal inflammatory factors (IL\u20106, TNF\u2010\u03b1, iNOS and COX\u20102) and MAPK pathway\u2010related proteins (p\u2010P38, p\u2010JNK, and p\u2010ERK) were increased both in DSS\u2010induced mouse intestinal tissue, but TEC inhibited expressions of inflammatory factors. The same increased trend was identified in LPS\u2010induced macrophages, but TEC improved macrophage inflammation, as evidenced by downregulation of inflammatory factors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>TEC mitigates IBD and LPS\u2010induced macrophage inflammation in mice via inhibiting MAPK signaling pathway.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.1077","type":"journal-article","created":{"date-parts":[[2024,5,9]],"date-time":"2024-05-09T13:59:26Z","timestamp":1715263166000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Tectorigenin inhibits inflammatory responses in murine\u00a0inflammatory bowel disease and LPS\u2010stimulated macrophages via inactivating MAPK signaling pathway"],"prefix":"10.1002","volume":"12","author":[{"given":"Hong","family":"Huang","sequence":"first","affiliation":[{"name":"Department of TCM and Integrated TCM and Western Medicine Hunan Provincial People's Hospital, The First Affiliated Hospital of Hunan Normal University Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanhui","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of TCM and Integrated TCM and Western Medicine Hunan Provincial People's Hospital, The First Affiliated Hospital of Hunan Normal University Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanghong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of TCM and Integrated TCM and Western Medicine Hunan Provincial People's Hospital, The First Affiliated Hospital of Hunan Normal University Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6353-1321","authenticated-orcid":false,"given":"Yi","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Liver and Gall Surgical Hunan Provincial People's Hospital, The First Affiliated Hospital of Hunan Normal University Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,5,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2016.3612"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v24.i28.3055"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20164061"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(17)32448-0"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.4110\/in.2017.17.1.25"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19072025"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13020-019-0245-x"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02976631"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1242\/bio.024562"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.6299"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2018.02.012"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2019.03.019"},{"issue":"7","key":"e_1_2_9_14_1","first-page":"632","article-title":"Urinary excretion of podocytes in patients with sepsis","volume":"13","author":"Watanabe T","year":"1999","journal-title":"Pediatr Nephrol"},{"key":"e_1_2_9_15_1","first-page":"622","article-title":"Andrographolide affects Th1\/Th2\/Th17 responses of peripheral blood mononuclear cells from ulcerative colitis patients","volume":"18","author":"Zhu Q","year":"2018","journal-title":"Mol Med Rep"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.2174\/1568010054022024"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2017.185"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.6299"},{"key":"e_1_2_9_19_1","unstructured":"LeiYU LiuJP ZhuangZX et al. 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