{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T03:59:33Z","timestamp":1789444773891,"version":"build-2803163510"},"reference-count":61,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T00:00:00Z","timestamp":1700611200000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32060805"],"award-info":[{"award-number":["32060805"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Cystic echinococcosis (CE), a zoonotic parasitic disease caused by <jats:italic>Echinococcus granulosus<\/jats:italic>, remains a public health and socioeconomic issue worldwide, making its prevention and treatment of vital importance. The aim of this study was to investigate changes in the intestinal microbiota of mice immunized with three peptide vaccines based on the recombinant antigen of <jats:italic>E. granulosus<\/jats:italic>, P29 (rEg.P29), with the hope of providing more valuable information for the development of vaccines against CE.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Three peptide vaccines, rEg.P29<jats:sub>T<\/jats:sub>, rEg.P29<jats:sub>B<\/jats:sub>, and rEg.P29<jats:sub>T\u2009+\u2009B<\/jats:sub>, were prepared based on rEg.P29, and a subcutaneous immunization model was established. The intestinal floras of mice in the different immunization groups were analyzed by 16\u2009S rRNA gene sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The intestinal microbiota analysis at both immunization time points revealed that Firmicutes, Bacteroidota, and Verrucomicrobiota were the predominant flora at the phylum level, while at the genus level, Akkermansia, unclassified_Muribaculaceae, Lachnospiraceae_NK4A136_group, and uncultured_rumen bacterium were the dominant genera. Some probiotics in the intestines of mice were significantly increased after immunization with the peptide vaccines, such as Lactobacillus_taiwanensis, Lactobacillus_reuteri,\u00a0Lachnospiraceae_NK4A136_group, Bacteroides_acidifaciens, and so forth. Meanwhile, some harmful or conditionally pathogenic bacteria were decreased, such as Turicibacter sanguinis, Desulfovibrio_fairfieldensis, Clostridium_sp, and so forth, most of which are associated with inflammatory or infectious diseases. Kyoto Encyclopaedia of Genes and Genomes enrichment analysis revealed that the differential flora were enriched in multiple metabolic pathways, primarily biological systems, human diseases, metabolism, cellular processes, and environmental information processing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In this study, we comprehensively analyzed and compared changes in the intestinal microbiota of mice immunized with three peptide vaccines as well as their related metabolic pathways, providing a theoretical background for the development of novel vaccines against <jats:italic>E. granulosus<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.1082","type":"journal-article","created":{"date-parts":[[2023,11,24]],"date-time":"2023-11-24T11:14:18Z","timestamp":1700824458000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Changes in intestinal flora of mice induced by rEg.P29 epitope peptide vaccines"],"prefix":"10.1002","volume":"11","author":[{"given":"Tingting","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Clinical Medicine Ningxia Medical University  Yinchuan China"},{"name":"Key Laboratory of Common Infectious Disease Prevention and Control in Ningxia  Yinchuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9095-0469","authenticated-orcid":false,"given":"Yongxue","family":"Lv","sequence":"additional","affiliation":[{"name":"Key Laboratory of Common Infectious Disease Prevention and Control in Ningxia  Yinchuan China"},{"name":"School of Basic Medical Sciences Ningxia Medical University  Yinchuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinqi","family":"Zhao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Common Infectious Disease Prevention and Control in Ningxia  Yinchuan China"},{"name":"Science and Technology Center of 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