{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T01:43:50Z","timestamp":1790819030367,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,12,13]],"date-time":"2023-12-13T00:00:00Z","timestamp":1702425600000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>There are new evidences that protein arginine methyltransferase 5 (PRMT5) is widely involved in the progression of various diseases, but its effect is unclear on Primary Sjogren's syndrome (pSS). The main purpose of this study is to explore the regulatory effect of PRMT5 on pSS and its potential mechanisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>CD40L treated CD19\u2009+\u2009B cells to construct a cell model of pSS. CCK\u20108 assay and Annexin V\u2010FITC\/PI kits were used to measure cell proliferation and apoptosis. ELISA assay was used to determine the contents of IL\u20106 and TNF\u2010\u03b1 in CD19\u2009+\u2009B cells. And commercial kits were used to detect the levels of immunoglobins (IgG, IgM, and IgA) in CD40L\u2010treated CD19\u2009+\u2009B cells. And successfully constructed a pSS mouse model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The results revealed an increase in the expression of PRMT5 in CD19\u2009+\u2009B cells from patients with pSS. After CD40L treatment, the knockdown of PRMT5 prominently decreased cell viability, the production level of immunoglobulins (IgG, IgM, and IgA), and the content of IL\u201010, increased the content of IL\u20106 and IL\u20108, and promoted the apoptosis of pSS CD19\u2009+\u2009B cells. Mechanistically, PRMT5 negatively regulated the RSAD2 and nuclear factor kappa\u2010B (NF\u2010\u03baB) signaling pathway. Furthermore, overexpression of RSAD2 and p65 significantly rescued the effect of PRMT5 knockdown on proliferation, immunoglobin production and secreting cytokines in CD40L\u2010treated CD19\u2009+\u2009B cells. More importantly, inhibition of PRMT5 significantly inhibited the symptoms of pSS mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Low\u2010expression of PRMT5 through inactivation of RSAD2\/NF\u2010\u03baB signalling pathway alleviates the hyperactivity of B cells, which may provide theoretical basis and potential therapeutic targets for clinical treatment of pSS.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.1102","type":"journal-article","created":{"date-parts":[[2023,12,13]],"date-time":"2023-12-13T05:56:26Z","timestamp":1702446986000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["RETRACTED: PRMT5 participates in B cell overactivation in patients with primary Sjogren's syndrome (pSS) through RSAD2\u2010mediated NF\u2010\u03baB signaling"],"prefix":"10.1002","volume":"11","author":[{"given":"Hong","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Rheumatology General Hospital of Ningxia Medical University Yinchuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0237-1748","authenticated-orcid":false,"given":"Jian","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Rheumatology General Hospital of Ningxia Medical University Yinchuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Rheumatology General Hospital of Ningxia Medical University Yinchuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Rheumatology General Hospital of Ningxia Medical University Yinchuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiantian","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Rheumatology General Hospital of Ningxia Medical University Yinchuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,12,13]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.survophthal.2019.10.004"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcp1702514"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/0961203318756287"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2792"},{"key":"e_1_2_10_6_1","first-page":"140","article-title":"Deregulation of microRNA expression in purified T and B lymphocytes from patients with primary Sjogren's syndrome","volume":"133","author":"Wang\u2010Renault S\u2010F","year":"2018","journal-title":"Ann Rheum Dis"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/life11101074"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.15698\/cst2020.08.228"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13075-020-02304-x"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2017.03.004"},{"key":"e_1_2_10_11_1","unstructured":"EbrahimiKH VowlesJ BrowneC et al. 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