{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T09:50:33Z","timestamp":1775555433685,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,12,6]],"date-time":"2023-12-06T00:00:00Z","timestamp":1701820800000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012286","name":"Deputy for Research and Technology, Kermanshah University of Medical Sciences","doi-asserted-by":"publisher","award":["3010723"],"award-info":[{"award-number":["3010723"]}],"id":[{"id":"10.13039\/501100012286","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The significance of T helper 17 (Th17) cells in the pathogenesis of rheumatoid arthritis (RA) has recently been demonstrated in many studies. Retinoic acid receptor\u2010related orphan receptor \u03b3t (ROR\u03b3t) is a transcription factor that is specifically involved in the generation of Th17 cells. Besides, the chemokine receptor CCR6, the receptor for CCL20, is characteristically expressed by these cells. Considering the pivotal roles of Th17 cells in RA pathogenesis, in this study, we assessed the gene expression of CCR6 and ROR\u03b3t in the peripheral blood leukocytes of new case RA patients. Also, we evaluated their association with anticyclic citrullinated peptide (anti\u2010CCP) antibodies and disease activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Forty\u2010five new case RA patients and 45 healthy persons have been recruited in this investigation. The gene expression of CCR6 and ROR\u03b3t was evaluated by quantitative real\u2010time PCR (qRT\u2010PCR), and anti\u2010CCP antibodies plasma levels were measured using the enzyme\u2010linked immunosorbent assay (ELISA) technique. Disease activity was measured according to the disease activity score\u201028 (DAS\u201028) formula.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The gene expression of CCR6 and ROR\u03b3t increased remarkably in new case RA patients compared to healthy controls (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05 and <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01, respectively). Moreover, there was a positive correlation between ROR\u03b3t gene expression and parameters, including gene expression of CCR6 (<jats:italic>p<\/jats:italic>\u2009=\u2009.001, <jats:italic>r<\/jats:italic>\u2009=\u2009.461), plasma levels of CCL20 (<jats:italic>p<\/jats:italic>\u2009=\u2009.0009, <jats:italic>r<\/jats:italic>\u2009=\u2009.477), ESR (<jats:italic>p<\/jats:italic>\u2009=\u2009.004, <jats:italic>r<\/jats:italic>\u2009=\u2009.419), DAS\u201028 (<jats:italic>p<\/jats:italic>\u2009=\u2009.006, <jats:italic>r<\/jats:italic>\u2009=\u2009.402), anti\u2010CCP (<jats:italic>p<\/jats:italic>\u2009=\u2009.019, <jats:italic>r<\/jats:italic>\u2009=\u2009.346), and RF (<jats:italic>p<\/jats:italic>\u2009=\u2009.001, <jats:italic>r<\/jats:italic>\u2009=\u2009.451). Also, CCR6 gene expression was positively associated with the DAS\u201028 (<jats:italic>p<\/jats:italic>\u2009=\u2009.037, <jats:italic>r<\/jats:italic>\u2009=\u2009.310), plasma levels of anti\u2010CCP (<jats:italic>p<\/jats:italic>\u2009=\u2009.037, <jats:italic>r<\/jats:italic>\u2009=\u2009.312), and ESR (<jats:italic>p<\/jats:italic>\u2009=\u2009.029, <jats:italic>r<\/jats:italic>\u2009=\u2009.327).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Increased gene expression of CCR6 and ROR\u03b3t in peripheral blood leukocytes of new case RA patients may contribute to the exacerbation and pathogenesis of RA.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.1112","type":"journal-article","created":{"date-parts":[[2023,12,6]],"date-time":"2023-12-06T12:28:47Z","timestamp":1701865727000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Increased gene expression of CCR6 and ROR\u03b3t in peripheral blood cells of rheumatoid arthritis patients and their correlation with anti\u2010cyclic citrullinated peptide and disease activity"],"prefix":"10.1002","volume":"11","author":[{"given":"Seyed Askar","family":"Roghani","sequence":"first","affiliation":[{"name":"Student Research Committee, Medical School Kermanshah University of Medical Sciences  Kermanshah Iran"},{"name":"Immunology Department, Faculty of Medicine Kermanshah University of Medical Sciences  Kermanshah Iran"},{"name":"Medical Biology Research Center, Health Technology Institute Kermanshah University of Medical Sciences  Kermanshah Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5421-5280","authenticated-orcid":false,"given":"Ramin","family":"Lotfi","sequence":"additional","affiliation":[{"name":"Clinical Research Development Center, Tohid Hospital Kurdistan University of Medical Sciences  Sanandaj Iran"},{"name":"Lung Diseases and Allergy Research Center, Research Institute for Health Development Kurdistan University of Medical Sciences  Sanandaj Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masood Ghasemzade","family":"Soroush","sequence":"additional","affiliation":[{"name":"Student Research Committee, Medical School Kermanshah University of Medical Sciences  Kermanshah Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ali","family":"Khorasanizadeh","sequence":"additional","affiliation":[{"name":"Student Research Committee, School of Medicine Ahvaz Jundishapur University of Medical Sciences  Ahvaz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Parisa","family":"Feizollahi","sequence":"additional","affiliation":[{"name":"Immunology Department, Faculty of Medicine Kermanshah University of Medical Sciences  Kermanshah Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shirin","family":"Assar","sequence":"additional","affiliation":[{"name":"Clinical 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