{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T11:59:59Z","timestamp":1791979199860,"version":"4.4.0"},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,2,2]],"date-time":"2024-02-02T00:00:00Z","timestamp":1706832000000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T00:00:00Z","timestamp":1706745600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100009102","name":"Education Department of Jiangxi Province","doi-asserted-by":"publisher","award":["GJJ201505"],"award-info":[{"award-number":["GJJ201505"]}],"id":[{"id":"10.13039\/501100009102","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp; Disease"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Neuropathic pain (NP) is a chronic pathological pain that affects the quality of life and is a huge medical burden for affected patients. In this study, we aimed to explore the effects of secreted phosphoprotein 1 (SPP1) on NP.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We established a chronic constriction injury (CCI) rat model, knocked down SPP1 via an intrathecal injection, and\/or activated the extracellular signal\u2010regulated kinase (ERK) pathway with insulin\u2010like growth factor 1 (IGF\u20101) treatment. Pain behaviors, including paw withdrawal threshold (PWT), paw withdrawal latency (PWL), lifting number, and frequency, were assessed. After sacrificing rats, the L4\u2010L5 dorsal root ganglion was collected. Then, SPP1 levels were determined using quantitative polymerase chain reaction (qPCR) and western blot analysis. The levels of interleukin (IL)\u20101\u03b2, tumor necrosis factor (TNF)\u2010\u03b1, IL\u20106, IL\u201010, epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), and transforming growth factor (TGF)\u2010\u03b2 were determined using qPCR and enzyme\u2010linked immunosorbent assay. The levels of ERK pathway factors were determined via western blot analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We found that CCI decreased PWT and PWL, increased the lifting number and frequency, and upregulated SPP1 levels. The loss of SPP1 reversed these CCI\u2010induced effects. Additionally, CCI upregulated IL\u20101\u03b2, TNF\u2010\u03b1, IL\u20106, EGF, and VEGF levels, downregulated TGF\u2010\u03b2 levels, and activated the ERK pathway, while silencing of SPP1 abrogated these CCI\u2010induced effects. Moreover, IGF\u20101 treatment reversed the effects of SPP1 loss.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The data indicate that silencing SPP1 attenuates NP via inactivation of the ERK pathway, suggesting that SPP1 may be a promising target for NP treatment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/iid3.1132","type":"journal-article","created":{"date-parts":[[2024,2,2]],"date-time":"2024-02-02T07:32:40Z","timestamp":1706859160000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Silencing of secreted phosphoprotein 1 attenuates sciatic nerve injury\u2010induced neuropathic pain: Regulating extracellular signal\u2010regulated kinase and neuroinflammatory signaling pathways"],"prefix":"10.1002","volume":"12","author":[{"given":"Haiyu","family":"Xie","sequence":"first","affiliation":[{"name":"Department of Anesthesiology The First Affiliated Hospital of Gannan Medical University  Ganzhou City Jiangxi Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology The First Affiliated Hospital of Gannan Medical University  Ganzhou City Jiangxi Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoling","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology The First Affiliated Hospital of Gannan Medical University  Ganzhou City Jiangxi Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enfu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology The First Affiliated Hospital of Gannan Medical University  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