{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T14:07:50Z","timestamp":1788962870090,"version":"build-2803163510"},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,2,14]],"date-time":"2024-02-14T00:00:00Z","timestamp":1707868800000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T00:00:00Z","timestamp":1706745600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp; Disease"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Celiac disease (CD) is a chronic autoimmune disorder characterized by an abnormal immune response to gluten, a protein found in wheat, barley, and rye. It is well established that the integrity of epithelial tight junctions (TJs) and adherens junctions (AJs) plays a crucial role in the pathogenesis of CD. These junctional complexes contribute to the apical\u2013basal polarity of the intestinal epithelial cells, which is crucial for their proper functioning.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Sixty CD subjects, and 50 controls were enrolled in the current study. Mucosal samples were obtained from the distal duodenum, total RNA was extracted and complementary DNA was synthesized. The relative expression levels of the desired genes were evaluated by quantitative real\u2010time polymerase chain reaction based on \u0394\u0394\n                      <jats:italic>C<\/jats:italic>\n                      <jats:sub>\n                        <jats:italic>t<\/jats:italic>\n                      <\/jats:sub>\n                      method. The gene\u2013gene interaction network was also constructed using GeneMANIA.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      <jats:italic>CRB3<\/jats:italic>\n                      (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u2009.0005),\n                      <jats:italic>LKB1<\/jats:italic>\n                      (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.0001), and\n                      <jats:italic>SCRIB<\/jats:italic>\n                      (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u2009.0005) had lower expression in CD patients compared to controls, while\n                      <jats:italic>PRKCZ<\/jats:italic>\n                      expression did not differ between groups (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&gt;\u2009.05).\n                      <jats:italic>CRB3<\/jats:italic>\n                      represented a significant diagnostic value for differentiating CD patients from the control group (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u2009.02).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The aim of the current study was to evaluate the changes in the mRNA expression levels of\n                      <jats:italic>SCRIB<\/jats:italic>\n                      ,\n                      <jats:italic>PRKCZ<\/jats:italic>\n                      ,\n                      <jats:italic>LKB1<\/jats:italic>\n                      , and\n                      <jats:italic>CRB3<\/jats:italic>\n                      genes in the small intestinal biopsy samples of CD patients in comparison to the healthy control subjects. Our data uncover the importance of polarity\u2010related genes (especially\n                      <jats:italic>CRB3<\/jats:italic>\n                      ) in CD pahtomechanism, that may facilitate the planning of the future studies looking for finding innovative diagnostic and therapeutic strategies for CD.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/iid3.1186","type":"journal-article","created":{"date-parts":[[2024,2,14]],"date-time":"2024-02-14T06:53:24Z","timestamp":1707893604000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Intestinal mRNA expression analysis of polarity\u2010related genes identified the discriminatory ability of CRB3 as a diagnostic marker for celiac disease"],"prefix":"10.1002","volume":"12","author":[{"given":"Tannaz","family":"Taraz","sequence":"first","affiliation":[{"name":"Department of Genetics, Faculty of Advanced Science and Technology, Tehran 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