{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T22:06:30Z","timestamp":1790978790957,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T00:00:00Z","timestamp":1710288000000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Diabetic cardiomyopathy (DCM) represents a major cause of heart failure and a large medical burden worldwide. This study screened the potentially regulatory targets of DCM and analyzed their roles in high glucose (HG)\u2010induced cardiomyocyte injury.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Through GEO database, we obtained rat DCM expression chips and screened differentially expressed genes. Rat cardiomyocytes (H9C2) were induced with HG. 3\u2010hydroxy\u20103\u2010methylglutarylcoenzyme A synthase 2 (Hmgcs2) and microRNA (miR)\u2010363\u20105p expression patterns in cells were measured by real\u2010time quantitative polymerase chain reaction or Western blot assay, with the dual\u2010luciferase assay to analyze their binding relationship. Then, 3\u2010(4,5\u2010dimethyl\u20102\u2010thiazolyl)\u20102,5\u2010diphenyl\u20102\u2010H\u2010tetrazolium bromide assay, lactate dehydrogenase assay, terminal deoxynucleotidyl transferase dUTP nick end labeling assay, enzyme\u2010linked immunosorbent assay, and various assay kits were applied to evaluate cell viability, cytotoxicity, apoptosis, inflammation responses, and oxidative burden.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Hmgcs2 was the vital hub gene in DCM. Hmgcs2 was upregulated in HG\u2010induced cardiomyocytes. Hmgcs2 downregulation increased cell viability, decreased TUNEL\u2010positive cell number, reduced HG\u2010induced inflammation and oxidative stress. miR\u2010363\u20105p is the upstream miRNA of Hmgcs2. miR\u2010363\u20105p overexpression attenuated HG\u2010induced cell injury.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Hmgcs2 had the most critical regulatory role in DCM. We for the first time reported that miR\u2010363\u20105p inhibited Hmgcs2 expression, thereby alleviating HG\u2010induced cardiomyocyte injury.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.1191","type":"journal-article","created":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T13:48:48Z","timestamp":1710337728000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Hmgcs2 is the hub gene in diabetic cardiomyopathy and is negatively regulated by Hmgcs2, promoting high glucose\u2010induced cardiomyocyte injury"],"prefix":"10.1002","volume":"12","author":[{"given":"Ying","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Endocrinology The Second Affiliated Hospital of Shandong First Medical University  Tai'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li\u2010Feng","family":"Ping","sequence":"additional","affiliation":[{"name":"Department of General Medicine The Second 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