{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T15:44:01Z","timestamp":1790178241142,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T00:00:00Z","timestamp":1711497600000},"content-version":"vor","delay-in-days":26,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["20YF1445300"],"award-info":[{"award-number":["20YF1445300"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The dosage of ovalbumin (OVA) during the sensitization stage is considered a crucial factor in the development of airway hyperresponsiveness (AHR). However, the inconsistent dosages of sensitizing OVA used in current studies and the lack of research on their impact on AHR are notable limitations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We examined the impact of increasing sensitizing doses of OVA in a murine asthma model, which entailed initial sensitization with OVA followed by repeated exposure to OVA aerosols. BALB\/c mice were primed with doses of OVA (0, 10, 20, 50, and 100\u2009\u03bcg) plus 1\u2009mg Alum on Days 0 and 7, and were challenged with OVA aerosols (10\u2009mg\/mL for 30\u2009min) between Days 14 and 17. Antigen\u2010induced AHR to methacholine (MCh), as well as histological changes, eosinophilic infiltration, and epithelial injury were assessed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The result indicated that there are striking OVA dose\u2010related differences in antigen\u2010induced AHR to MCh. The most intense antigen\u2010induced AHR to MCh was observed with sensitization at 50\u2009\u03bcg, while weaker responses were seen at 10, 20, and 100\u2009\u03bcg. Meanwhile, there was a significant increase in eosinophil count with sensitization at 50\u2009\u03bcg. The changes of AHR were correlated with total cells count, lymphocytes count, eosinophils count, and basophils count in bronchoalveolar lavage fluid; however, it did not correlate with histological changes such as cellular infiltration into bronchovascular bundles and goblet cell hyperplasia of the bronchial epithelium.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Overall, this study demonstrated that sensitization with 50\u2009\u03bcg of OVA resulted in the most significant AHR compared to other dosages. These findings may offer valuable insights for future research on mouse asthma modeling protocols.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/iid3.1225","type":"journal-article","created":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T07:21:54Z","timestamp":1711524114000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Effects of increasing sensitizing doses of ovalbumin on airway hyperresponsiveness in asthmatic mice"],"prefix":"10.1002","volume":"12","author":[{"given":"Yan\u2010Jiao","family":"Chen","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Biology, Shanghai Research Institute of Acupuncture and Meridian Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Shanghai P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Yuan","sequence":"additional","affiliation":[{"name":"Deparment of Acupuncture and Moxibustion Guanghua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine Shanghai P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Peng","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology, Shanghai Research Institute of Acupuncture and Meridian Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Shanghai P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin\u2010Yi","family":"Dong","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology, Shanghai Research Institute of Acupuncture and Meridian Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Shanghai P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Dong","family":"Xu","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology, Shanghai Research Institute of Acupuncture and Meridian Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Shanghai P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanghai University of Traditional Chinese Medicine Shanghai P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8259-6359","authenticated-orcid":false,"given":"Yong\u2010Qing","family":"Yang","sequence":"additional","affiliation":[{"name":"Shanghai University of Traditional Chinese Medicine Shanghai P.R. 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